Cytotoxicity activity of biotransformed ethyl p-methoxycinnamate by Aspergillus niger

• Main Authors: Omar, Muhammad Nor, Hasali, Nor Hazwani, Yarmo, Mohd Ambar
• Format: Article
• Language: English; English; English
• Published: Oriental Scientific Publishing Company 2016
• Subjects: QD Chemistry
• Online Access: http://irep.iium.edu.my/54229/1/PROF%20NO%20OJC_Vol32_No5_p_2731-2734-2.pdf; http://irep.iium.edu.my/54229/7/54229_Cytotoxicity%20Activity%20of%20Biotransformed%20Ethyl_WOS.pdf; http://irep.iium.edu.my/54229/8/54229_Cytotoxicity%20Activity%20of%20Biotransformed%20Ethyl_SCOPUS.pdf; http://irep.iium.edu.my/54229/; http://www.orientjchem.org/vol32no5/cytotoxicity-activity-of-biotransformedethyl-p-methoxycinnamate-by-aspergillusniger/

The extraction of Kaempferia galanga rhizome using steam distillation and supercritical fluid extraction (SFE) was carried out. After fractionation, the major compound of the K. galanga extract, ethyl p-methoxycinnamate (EPMC) was transformed using Aspergillus niger into ethyl p-hydroxycinnamate (EPHC). The biological anticancer activity of EPMC and its biotransformed product (EPHC) was established by cytotoxicity activity on the human breast cancer (MCF-7) cell line using MTT assay. Ethyl p-hydroxycinnamate (EPHC) was most cytotoxic against MCF-7 at 1000 μg/mL where percentage of cell viability was 9.87 %, while IC50 was 340 μg/mL. EPHC showed slightly higher cytotoxicity activity compared to EPMC. The results of this study show that the biotransformation process was able to produce a metabolite (EPHC) with higher cytotoxicity activity compared to its parent compound (EPMC).