Molecular cloning and optimization for high level expression of cold-adapted serine protease from antarctic yeast glaciozyma antarctica PI12

• Main Authors: Alias, Norsyuhada, Ahmad Mazian, Mu’adz, Salleh, Abu Bakar, Basri, Mahiran, Raja Abd. Rahman, Raja Noor Zaliha
• Format: Article
• Language: English
• Published: Hindawi Publishing Corporation 2014
• Subjects: QR Microbiology
• Online Access: http://irep.iium.edu.my/53050/1/197938.pdf; http://irep.iium.edu.my/53050/; https://www.hindawi.com/journals/er/2014/197938/; http://dx.doi.org/10.1155/2014/197938

Psychrophilic basidiomycete yeast, Glaciozyma antarctica strain PI12, was shown to be a protease-producer. Isolation of the PI12 protease gene from genomic and mRNA sequences allowed determination of 19 exons and 18 introns. Full-length cDNA of PI12 protease gene was amplified by rapid amplification...