Tualang honey improves non alcoholic steatohepatitis animal model
Introduction: Non-alcoholic Steatohepatitis (NASH) is an emerging chronic liver disease with limited therapy available. Studies utilizing animal models induced with cholesterol diet ranging from 1 -2% are hampered by inconsistent yield of NASH features. Therefore, we aimed to establish a NASH ani...
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| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English English |
| Published: |
2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/52995/12/Medical%20Research%20Symposium_brochure.pdf http://irep.iium.edu.my/52995/13/52995_tualang%20honey.pdf http://irep.iium.edu.my/52995/ http://iiumedic.net/imjm/v1/ |
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| Summary: | Introduction: Non-alcoholic Steatohepatitis (NASH) is an emerging chronic liver disease
with limited therapy available. Studies utilizing animal models induced with cholesterol
diet ranging from 1 -2% are hampered by inconsistent yield of NASH features. Therefore,
we aimed to establish a NASH animal model utilizing 12% cholesterol diet (CD) and to
investigate the effects of Tualang honey (TH) known for its anti-inflammatory and antioxidative properties in this model. Methods: Twenty-four Sprague-Dawley rats were
divided into 2 groups (12% CD and standard diet) and were fed for 6 weeks. Following the
establishment of NASH, the rats in the 12% CD group were subsequently divided into 3
groups. The first group was continued with only 12% CD. In the other 2 groups in addition
to the 12% CD they were given TH treatment at different concentrations (1.2 and 2.4
g/kg/day) for 4 weeks. Blood biochemical analysis and histological assessment of liver
were subsequently performed. Results: The liver histological sections of the rats fed with
12% CD showed macrovesicular steatosis, ballooning degeneration with lobular and portal
inflammation. They also had increased serum alanine aminotransferase (ALT), total
cholesterol (TC), low density lipoprotein cholesterol (LDL-C), fasting insulin, HOMA-IR and
reduced high density lipoprotein cholesterol (HDL-C). Meanwhile, the TH treatment groups
exhibited significant improvement in both the NASH grading and activity scores. The ALT,
LDL-C, TC, triglyceride (TG), fasting insulin and HOMA-IR levels were reduced significantly.
Conclusions: The 12% CD was able to induce NASH in the animal model. Tualang honey
improved insulin sensitivity, dyslipidaemia, steatohepatitis.
KEYWORDS: NASH, cholesterol diet, Tualang Honey |
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