Cytotoxic activity of Luvunga scandens against human cancer cell lines

• Main Authors: Al Zikri, Putri Nurhidayah, Bakhtiar, M. Taher, Darnis, Deny Susanti, Arief Ichwan, Solachuddin Jauhari
• Format: Article
• Language: English; English; English
• Published: Penerbit UTM Press, Universiti Teknologi Malaysia. 2016
• Subjects: RS Pharmacy and materia medica
• Online Access: http://irep.iium.edu.my/52347/1/Journal%20Teknologi%20Luvunga.pdf; http://irep.iium.edu.my/52347/7/52347_Cytotoxic%20activity%20of%20Luvunga_WOS.pdf; http://irep.iium.edu.my/52347/8/52347_Cytotoxic%20activity%20of%20Luvunga_SCOPUS.pdf; http://irep.iium.edu.my/52347/; http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/7328/5836

Luvunga scandens belongs to the family of Rutaceae which usually inhabit tropical and moist environment. This plant is known as ‘Mengkurat Jakun’ among locals and used traditionally to treat fever and fatigue via decoction. Purpose: The aim of this study was to investigate the cytotoxic activity of the leaves and stems extracts of L. scandens extract. Methods: Extracts of the leaves and stems were obtained from sequential extraction procedures by various organic solvents. All extracts were subjected to cytotoxic study by 3-(4, 5-dimethylthaizol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Results: In in vitro cytotoxicity assay, all L. scandens extracts exhibited cytotoxicity against human breast adenocarcinoma (MCF-7) and human lung adenocarcinoma (A549) cell lines. The IC50 values of dichloromethane and methanol extracts from the leaves of L. scandens against MCF-7 cell line were 62.5 µg/mL and 88.0 µg/mL, respectively, whereas IC50 of methanol extract from stem was 81.0 µg/mL. All extracts were less active against A549 cell line where IC50 value were not be determined. Conclusion: The present findings revealed the potential of L. scandens as a cytotoxic agent against MCF-7 cell line. However, further studies should be planned to evaluate role of the plant in cytotoxic activity. Luvunga scandens adalah species tumbuhan tergolong dalam keluarga Rutaceae yang kebiasaannya mendiami persekitaran tropika dan lembap. Pokok ini dikenali sebagai ‘Mengkurat Jakun’ di kalangan penduduk tempatan dan digunakan secara tradisional untuk mengubati demam dan keletihan dengan meminum air rebusan. Kajian ini adalah bertujuan untuk menyiasat aktiviti ketoksikan ekstrak daripada daun dan batang pokok L. scandens. Ekstrak daripada daun dan batang telah diperoleh daripada prosedur pengekstrakan berurutan oleh pelbagai pelarut organik. Kesemua ekstrak telah diuji untuk pemeriksaan aktiviti ketoksikan menggunakan 3-(4, 5- dimethylthaizol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Dalam ujian ketoksikan, kesemua ekstrak dari L. scandens menunjukan ketoksikan terhadap sel payudara adenokarsinoma (MCF-7) dan sel epitelium paru-paru adenocarcinoma (A549). Nilai IC50 dari ekstrak diklorometana dan metanol daripada daun L. scandens terhadap MCF-7 sel adalah masing-masing 62.5 µg/mL dan 88.0 µg/mL, manakala ekstrak methanol daripada batang adalah 81.0 µg/mL. Semua ekstrak kurang aktif terhadap sel A549, dimana nilai IC50 tidak ditentukan. Kajian mendapati bahawa, L. scandens mempunyai potensi sebagai agen sitotoksik terhadap sel MCF-7. Walaubagaimanapun, kajian selanjutnya telah dirancang untuk menilai peranan tumbuhan dalam aktiviti sitotoksik.