Temperature effect on expression of soluble Endoglucanase I from Fusarium oxysporum using E. coli Lemo21:DE3

• Main Authors: Noor Batcha, Ibrahim Ali, Raja Izzuddin, Raja Hawa Nurathirah, Ahmad, Abdul Aziz, Mohd Salleh, Hamzah
• Format: Conference or Workshop Item
• Language: English
• Published: Kulliyah of Engineering, International Islamic University Malaysia 2016
• Subjects: TP248.13 Biotechnology
• Online Access: http://irep.iium.edu.my/51788/1/51788.pdf; http://irep.iium.edu.my/51788/; http://www.iium.edu.my/icbioe/2016/

Formation of inclusion bodies especially when expressing eukaryotic recombinant proteins in prokaryotic host such as E. coli is a challenge. Ability to express soluble proteins in E.coli will enable us to utilize all the benefits of E.coli as a host mainly the short doubling time. As few reports i...