Screening of thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake
Special consideration has been focused on the utilization of lipase enzyme as a suitable alternative method for biodiesel production, which is expected to be more stable and practical. However, the high production costs as well as low productivity have hindered the marketable production of biodies...
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Main Authors: | , , , |
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Format: | Conference or Workshop Item |
Language: | English |
Published: |
Kulliyah of Engineering, International Islamic University Malaysia
2016
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Subjects: | |
Online Access: | http://irep.iium.edu.my/51681/1/51681.pdf http://irep.iium.edu.my/51681/ |
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Summary: | Special consideration has been focused on the utilization of lipase enzyme as a suitable alternative method for
biodiesel production, which is expected to be more stable and practical. However, the high production costs
as well as low productivity have hindered the marketable production of biodiesel using the commercial lipase.
Isolation of lipases from filamentous fungi, particularly found in agricultural wastes has attracted special
interest due to their potential towards developing more sustainable and economical applications. Only lipase
possessing thermal stability and organic solvent tolerant can distinctively enhance the biodiesel production.
Therefore, in the present study, isolation of thermostable- organic solvent tolerant fungal strains from palm
kernel cake (PKC) were conducted based on their ability to grow at 45oC on YpSs (yeast extract + starch +
agar) media and in the presence of organic solvent. The isolates were screened based on the clearing zone
methods on phenol red as well as on tween 80 agar media. Four isolates were screened for potential lipase
production. These isolates were incubated under Solid State Fermentation (SSF) at 45oC for 7 days with PKC
as substrate. The most promising strain with the potential to produce lipase was PKC12B2, which showed
satisfactory enzyme activity. |
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