Formulation development and systematic optimization of production fish gelatine nanoparticles as new resource for drug delivery

Animal proteins have been chosen as promising material for producing nanoparticle and its applications have become popular on the drug delivery research area. Furthermore, gelatine nanoparticles is best biodegradable material, biocompatible, and chemical modification potential, due to its characteri...

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Main Authors: Subara, Deni, Jaswir, Irwandi, Noorbatcha, Ibrahim Ali, Al-Khatib, Ma'an Fahmi Rashid
Format: Proceeding Paper
Language:English
Published: Kulliyyah of Engineering, International Islamic University Malaysia 2016
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Online Access:http://irep.iium.edu.my/51515/1/51515_Formulation_Development_and_Systematic_Optimization.pdf
http://irep.iium.edu.my/51515/
http://www.iium.edu.my/icbioe/2016/
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spelling my.iium.irep.515152024-05-06T07:05:56Z http://irep.iium.edu.my/51515/ Formulation development and systematic optimization of production fish gelatine nanoparticles as new resource for drug delivery Subara, Deni Jaswir, Irwandi Noorbatcha, Ibrahim Ali Al-Khatib, Ma'an Fahmi Rashid TP248.13 Biotechnology Animal proteins have been chosen as promising material for producing nanoparticle and its applications have become popular on the drug delivery research area. Furthermore, gelatine nanoparticles is best biodegradable material, biocompatible, and chemical modification potential, due to its characteristic, gelatine nanoparticles are very promising in carrier system for drug delivery. Mostly the commercial gelatines are derived from mammalian sources, mainly porcine and bovine. Recently, fish gelatine has provided alternative resources for religious, safety and economic reason. In this present research, the fish gelatine was used as a material for production gelatine nanoparticles. The long-term goal of this study is to develop a nanoparticulate formulation based on halal gelatine, for delivery of fragile molecules such as protein and peptide drug. Fish gelatine nanoparticles were synthesized via a two-step desolvation method. Furthermore, the aim of the present study was to assess various formulation parameters in the first step from two step desolvation method to produce fish gelatine nanoparticles that have minimum in size. In the first step, high molecular weight (HMW) fraction content was optimized by SDS-PAGE. The nanoparticles formed were further characterized for mean size, size distribution using a Zetasizer nano, while the morphology of the particles was evaluated by field emission scanning electron microscope (FESEM). The smallest size of achieved nanoparticles was 227 nm which is suitable for drug delivery. The study indicated that a high fraction of HMW in first step could be obtained with gelatine concentration 9%, temperature 45 °C, centrifugation speed at 9700 rpm, and centrifugation time was 5 minute. The mechanistic of the optimum conditions for producing fish gelatine nanoparticles as well as their characterization are discussed. Kulliyyah of Engineering, International Islamic University Malaysia 2016 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/51515/1/51515_Formulation_Development_and_Systematic_Optimization.pdf Subara, Deni and Jaswir, Irwandi and Noorbatcha, Ibrahim Ali and Al-Khatib, Ma'an Fahmi Rashid (2016) Formulation development and systematic optimization of production fish gelatine nanoparticles as new resource for drug delivery. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Subara, Deni
Jaswir, Irwandi
Noorbatcha, Ibrahim Ali
Al-Khatib, Ma'an Fahmi Rashid
Formulation development and systematic optimization of production fish gelatine nanoparticles as new resource for drug delivery
description Animal proteins have been chosen as promising material for producing nanoparticle and its applications have become popular on the drug delivery research area. Furthermore, gelatine nanoparticles is best biodegradable material, biocompatible, and chemical modification potential, due to its characteristic, gelatine nanoparticles are very promising in carrier system for drug delivery. Mostly the commercial gelatines are derived from mammalian sources, mainly porcine and bovine. Recently, fish gelatine has provided alternative resources for religious, safety and economic reason. In this present research, the fish gelatine was used as a material for production gelatine nanoparticles. The long-term goal of this study is to develop a nanoparticulate formulation based on halal gelatine, for delivery of fragile molecules such as protein and peptide drug. Fish gelatine nanoparticles were synthesized via a two-step desolvation method. Furthermore, the aim of the present study was to assess various formulation parameters in the first step from two step desolvation method to produce fish gelatine nanoparticles that have minimum in size. In the first step, high molecular weight (HMW) fraction content was optimized by SDS-PAGE. The nanoparticles formed were further characterized for mean size, size distribution using a Zetasizer nano, while the morphology of the particles was evaluated by field emission scanning electron microscope (FESEM). The smallest size of achieved nanoparticles was 227 nm which is suitable for drug delivery. The study indicated that a high fraction of HMW in first step could be obtained with gelatine concentration 9%, temperature 45 °C, centrifugation speed at 9700 rpm, and centrifugation time was 5 minute. The mechanistic of the optimum conditions for producing fish gelatine nanoparticles as well as their characterization are discussed.
format Proceeding Paper
author Subara, Deni
Jaswir, Irwandi
Noorbatcha, Ibrahim Ali
Al-Khatib, Ma'an Fahmi Rashid
author_facet Subara, Deni
Jaswir, Irwandi
Noorbatcha, Ibrahim Ali
Al-Khatib, Ma'an Fahmi Rashid
author_sort Subara, Deni
title Formulation development and systematic optimization of production fish gelatine nanoparticles as new resource for drug delivery
title_short Formulation development and systematic optimization of production fish gelatine nanoparticles as new resource for drug delivery
title_full Formulation development and systematic optimization of production fish gelatine nanoparticles as new resource for drug delivery
title_fullStr Formulation development and systematic optimization of production fish gelatine nanoparticles as new resource for drug delivery
title_full_unstemmed Formulation development and systematic optimization of production fish gelatine nanoparticles as new resource for drug delivery
title_sort formulation development and systematic optimization of production fish gelatine nanoparticles as new resource for drug delivery
publisher Kulliyyah of Engineering, International Islamic University Malaysia
publishDate 2016
url http://irep.iium.edu.my/51515/1/51515_Formulation_Development_and_Systematic_Optimization.pdf
http://irep.iium.edu.my/51515/
http://www.iium.edu.my/icbioe/2016/
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score 13.159267