Solid state bioconversion of oil palm empty fruit bunches for cellulase enzyme production using a rotary drum bioreactor

• Main Authors: Alam, Md. Zahangir, Al-Mamun, Abdullah, Qudsieh, Isam Y., Muyibi, Suleyman Aremu, Mohd. Salleh, Hamzah, Omar, Nor Marina
• Format: Article
• Language: English
• Published: Elsevier Science 2009
• Subjects: QP Physiology; TP Chemical technology; TP248.13 Biotechnology
• Online Access: http://irep.iium.edu.my/5058/1/Cellulase-solid-state-BEJ.pdf; http://irep.iium.edu.my/5058/; http://dx.doi.org/10.1016/j.bej.2009.03.010

Bioconversion of oil palm lignocellulosic biomass in the form of empty fruit bunches (EFB) as the major substrate into cellulase enzyme was studied in the laboratory. A fungal strain, Trichoderma harzianum T2008 was used to evaluate the solid state bioconversion of EFB for cellulase production. The study was conducted in two systems: an Erlenmeyer flask (EF, 500 mL) and a horizontal rotary drum bioreactor (RDB, 50 L), designed and fabricated locally. The highest cellulase activity on the fourth day of fermentation in the EF was 8.2 filter paper activity (FPA)/gram dry solids (gds) of EFB, while its activity from the RDB was 10.1 FPA/gds on the second day of fermentation. Glucosamine, reducing sugar and pH were also determined to evaluate the substrate uptake and growth conditions during the entire fermentation period.