Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactideco- glycolide) microspheres

• Main Authors: Ansary, Rezaul Haque, Rahman, Md. Mokhlesur, Awang, Mohamed, Katas, Haliza, Ab. Hadi, Hazrina, Mohamed, Farahidah, Doolaanea, Abd Almonem, Yunus, Kamaruzzaman
• Format: Article
• Language: English; English
• Published: Springer 2016
• Subjects: QD Chemistry
• Online Access: http://irep.iium.edu.my/49239/4/49239-article.pdf; http://irep.iium.edu.my/49239/10/49239_Preparation%2C%20characterization%20and%20in%20vitro%20release%20study%20of%20BSA_scopus.pdf; http://irep.iium.edu.my/49239/; http://link.springer.com/article/10.1007%2Fs12272-016-0710-3

The aim of this study was to prepare a model protein, bovine serum albumin (BSA) loaded double-walled microspheres using a fast degrading glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu- PLGA) and a moderate-degrading carboxyl-terminated PLGA polymers to reduce the initial burst release and to eliminate the lag phase from the release profile of PLGA microspheres. The double-walled microspheres were prepared using a modified water-in-oil-in-oil-in-water (w/o/o/w) method and singlepolymer microspheres were prepared using a conventional water-in-oil-in-water (w/o/w) emulsion solvent evaporation method. The particle size, morphology, encapsulation efficiency, thermal properties, in vitro drug release and structural integrity of BSA were evaluated in this study. Double-walled microspheres prepared with Glu-PLGA and PLGA polymers with a mass ratio of 1:1 were non-porous, smooth-surfaced, and spherical in shape. A significant reduction of initial burst release was achieved for the double-walled microspheres compared to single-polymer microspheres. In addition, microspheres prepared using Glu- PLGA and PLGA polymers in a mass ratio of 1:1 exhibited continuous BSA release after the small initial burst without any lag phase. It can be concluded that the double-walled microspheres made of Glu-PLGA and PLGA polymers in a mass ratio of 1:1 can be a potential delivery system for pharmaceutical proteins.