Induction of apoptosis and oxidative stress in estrogen receptor-negative breast cancer, MDA-MB231 cells, by ethanolic mango seed extract

• Main Authors: Hussah Abdullah, Al-Shwyeh, Sabo Mohammed, Abdulkarim, Rasedee, Abdullah, Mirghani, Mohamed Elwathig Saeed, Al-Qubaisi, Mothanna Sadiq
• Format: Article
• Language: English; English
• Published: BioMed Central 2015
• Subjects: Q Science (General)
• Online Access: http://irep.iium.edu.my/48564/1/Mango_%28B_cancer%29_12906_2015_BMC.pdf; http://irep.iium.edu.my/48564/4/48564_wos-scopus.pdf; http://irep.iium.edu.my/48564/; http://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-015-0575-x

In this study, the effect of mango kernel extract in the induction of apoptosis of the breast cancer (MDA-MB-231) cell line was examined. This is an attempt to discover alternatives to current therapeutic regimes in the treatment of breast cancers. Methods: The pro-apoptotic markers, Bax, cytochrome c, caspases-. -8 and -9, and anti-apoptotic markers, Bcl-2, p53 and glutathione were determined in MDA-MB231 cells treated for 12 and 24h with mango kernel extract. Results: The results showed that the extract produced a time- and dose-dependent increases in pro-apoptotic proteins and oxidative stress markers with a corresponding decrease in anti-apoptotic markers. Conclusions: Based on the findings, mango kernel extract modulates redox balance in MDA-MB-231 breast cancer cells with a tendency for apoptotic cell death. The changes observed in this study may collectively underlie the basis for the cell death induced in MDA-MB-231 breast cancer cells by mango kernel extract. Thus, mango kernel extract has potential to be developed into an antibreast cancer mixture, and hence these results are worth studying further.