Performance of β-glucosidase immobilized on calcium alginate beads

• Main Authors: Jameel, Ahmed Tariq, Yusof, Faridah, Johana, Syaira
• Format: Conference or Workshop Item
• Language: English; English
• Published: 2013
• Subjects: TP Chemical technology; TP155 Chemical engineering
• Online Access: http://irep.iium.edu.my/48525/1/ICBIOE-2013-PERFORMANCE_OF_%CE%B2-GLUCOSIDASE_IMMOBILIZED_ON_CALCIUM.pdf; http://irep.iium.edu.my/48525/4/1175_Acceptance.pdf; http://irep.iium.edu.my/48525/; http://www.iium.edu.my/icbioe/2013

Performance of immobilized β-glucosidase obtained from almonds (EC 3.2.1.21) on calcium alginate beads was studied by measuring the activity of the enzyme in terms of the generation of pnitrophenol from the hydrolysis of p-nitrophenyl-β-D-glucopyranoside (PNPG).The immobilized enzyme activity was compared with soluble enzyme and was found to decrease by 36.6% albeit with an increased operational stability in terms of easy recovery from the finished product, recurrent use and scale-up in various reactor configurations. The hydrolysis rate data exhibit Michaelis-Menten kinetics. The Michaelis constant were determined using Langmuir linearized plot which obtained vmax and Km as 20.88 μmol/mL.min and 0.0125 mol/L respectively. However, the immobilized β-Glucosidase did not show a sufficiently good operational stability on reuse. Keywords: Immobilized enzyme, enzyme activity, Michaelis-Menten kinetics, β-glucosidase, pnitrophenyl- β-D-glucopyranoside (PNPG), alginate gel