Selection of potentially optimized trypsin variant with a more hydrophobic cluster active site via a phage display approach

• Main Authors: Abdul Kudos, Muhammad Badri, Gissel, Sabrina, Bordusa, Frank
• Format: Conference or Workshop Item
• Language: English; English
• Published: 2015
• Subjects: TP248.13 Biotechnology
• Online Access: http://irep.iium.edu.my/48397/1/Badri%20KOP.pdf; http://irep.iium.edu.my/48397/2/Poster_badri_ACB2015.pdf; http://irep.iium.edu.my/48397/

Protein optimization can generally be obtained by rational enzyme design and directed evolution. Phage display has been used as a method for directed evolution. Trypsin has been studied as a biocatalyst in peptide synthesis despite its usual function in hydrolyzing peptide bonds. The objective of th...