Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer

• Main Authors: Bahrudin, Nik Adlin, Al-Khatib, Ma'an Fahmi Rashid, Nasef, Mohamed Mahmoud
• Other Authors: Haneef, Sayed Sikandar Shah
• Format: Proceeding Paper
• Language: English
• Published: Kulliyyah of Engineering, International Islamic University Malaysia 2016
• Subjects: TP248.13 Biotechnology
• Online Access: http://irep.iium.edu.my/47871/1/47871_Optimization_of_Lipase_Immobilization.pdf; http://irep.iium.edu.my/47871/; http://www.iium.edu.my/icbioe/2016/

Lipase from wheat germ was immobilized by covalent binding on a nylon -6- grafted with PGMA. This polymer was successfully activated with diethyl amine to intact with lipase enzyme molecule. Response surface methodology (RSM) was applied to model and optimize immobilization conditions for maximum...