Investigation on the difference expression of type IV collagen α1(IV)- α6(IV) chain mrna in normal fibroblast and in skin cell malignant melanoma

• Main Authors: Mohammad, Mardhiah, Mat Yusof, Afzan, Kaja Mohideen, Syahirah, Syed Bahrom, Sharifah Norbaizura, Roshdi, Ridhwan, Parman, Adibah
• Format: Article
• Language: English
• Published: Universiti Teknologi Malaysia 2015
• Subjects: Q Science (General)
• Online Access: http://irep.iium.edu.my/46358/1/6754-18543-1-SM_%281%29MAR.pdf; http://irep.iium.edu.my/46358/; http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/6754

Collagen IV is the major basement membrane protein that influences adhesion, proliferation, and migration of cells. The collagen composed of a network chains a1 to a6. The characterization of this collagen IV will correlates the relationship of collagen gene expression and cancer. This is important in order to provide more detailed understanding of the expression of collagen in tumor cells. . The aim of this study is to determine the a1 to a6 (IV) mRNA expression in the cell lines obtained from skin and melanoma cell. To investigate the mRNA expression, the RNA was extracted from the fibroblast and melanoma (A375) cell lines. The RNA was subjected to reverse transcription and then synthesized. The mRNA expression levels were measured using real time PCR with related to internal control, GAPDH. The study identified that a1, a2, a4, a5 and a6 of the a1-a6 (IV) were expressed in skin fibroblast. This corresponds to the a1a1a2 and a5a5a6 networks. However, in melanoma cell lines the collagen IV a2, a4, a5 and a6 mRNA was observed in low level compared to a1 and this suggested that the tumor has affected the expression of collagen and basement membrane of the cell.