Trypanosomatid phosphoglycerate mutases have multiple conformational and oligomeric states

• Main Authors: Blackburn, Elizabeth A., Ahmad Fuad, Fazia Adyani, Morgan, Hugh P., Nowicki, Matthew W., Wear, Martin A., Michels, Paul A.M., Fothergill-Gilmore, Linda A., Walkinshaw, Malcolm D.
• Format: Article
• Language: English
• Published: ELSEVIER 2014
• Subjects: Q Science (General)
• Online Access: http://irep.iium.edu.my/45465/1/%2BBlackburn_2014_BBRC.pdf; http://irep.iium.edu.my/45465/; http://www.journals.elsevier.com/biochemical-and-biophysical-research-communications/; http://dx.doi.org/10.1016/j.bbrc.2014.06.113

Three structurally distinct forms of phosphoglycerate mutase from the trypanosomatid parasite Leishmania mexicana were isolated by standard procedures of bacterial expression and purification. Analytical size-exclusion chromatography coupled to a multi-angle scattering detector detected two monomeri...