Micromachined adjustable hydrogel stamper for soft printing of biomolecules with adjustable-feature dimensions.
We report on the development of a hydrogel stamper with built-in reservoirs for soft-printing of biomolecules with adjustable feature dimensions. The stamper consists of potassium hydroxide (KOH)-etched silicon cavities onto which biomolecule-soaked low cross-linked density hydrogel with large pores...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English English English |
| Published: |
American Chemical Society
2009
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/44326/1/salim2009.pdf http://irep.iium.edu.my/44326/7/44326_Micromachined%20hydrogel%20_scopus.pdf http://irep.iium.edu.my/44326/8/44326_Micromachined%20hydrogel%20_wos.pdf http://irep.iium.edu.my/44326/ http://pubs.acs.org/journal/ancham |
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| Summary: | We report on the development of a hydrogel stamper with built-in reservoirs for soft-printing of biomolecules with adjustable feature dimensions. The stamper consists of potassium hydroxide (KOH)-etched silicon cavities onto which biomolecule-soaked low cross-linked density hydrogel with large pores and low mechanical strength is loaded. Application of weight to the top surface allows for a controllable protrusion of hydrogel from the opposite nozzles. Such protrusion combined with a suitable spacer between the stamper and a substrate provides a means for printing features with dimensions depending on the applied weight. Utilizing the above method, we successfully stamped bovine serum albumin conjugated with fluorescein isothiocyanate (BSA-FITC) model proteins on hydrophilic silicon substrates with a feature dimension ratio of 20:1 using a single stamper. |
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