Effect of lectin from artocarpus heterophyllus seed on cancer cell lines
The objective of this paper to present cytotoxic effect of jacalin, a purified protein and crude protein from A.heterophyllus seed against cancer cell, human breast cancer (MCF7) and non-small lung carcinoma (H1299). The extraction and purification were carried out by phosphate buffer precipitatio...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/43075/4/lectin.pdf http://irep.iium.edu.my/43075/ http://www.jolst.net/ |
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| Summary: | The objective of this paper to present cytotoxic effect of jacalin, a purified protein and crude
protein from A.heterophyllus seed against cancer cell, human breast cancer (MCF7) and non-small lung
carcinoma (H1299). The extraction and purification were carried out by phosphate buffer precipitation
and reverse miceller, respectively. MTT and trypan blue exclusion methods were performed to study
the cytotoxic activity. Jacalin and crude protein was compared in cytotoxic activity against MCF7 and
H1299 cancer cells and the result showed jacalin more effective than crude protein, 64.73 and 75.54
percent for MCF7 and 67.71 and 68.39 percent for H1299, respectively. Jacalin also found very active
against MCF7 compared to H1299 cancer cells and expected IC50 for both cancer cells would be
reached if concentration of jacalin will extended in cytotoxic activity analysis.
Keywords: Artocarpus heterophyllus, jacalin, crude protein, cytotoxicity,cancer cell, MCF7, H1299 |
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