High performance liquid chromatographic determination of mefenamic acid in human plasma using UV vis detector

• Main Authors: Helal Uddin, A.B.M., Mohamad, Huda Jamilah, Alaama, Mohammed, Amiruddin, Noor Syafawati
• Format: Article
• Language: English; English
• Published: Academic Sciences 2014
• Subjects: QD Chemistry; RS Pharmacy and materia medica
• Online Access: http://irep.iium.edu.my/40411/1/HIGH_PERFORMANCE_LIQUID_CHROMATOGRAPHIC_DETERMINATION_OF_MEFENAMIC_ACID_IN_HUMAN_PLASMA_USING_UV_VIS_DETECTOR.pdf; http://irep.iium.edu.my/40411/4/40411_High%20performance%20liquid%20chromatographic.SCOPUSpdf.pdf; http://irep.iium.edu.my/40411/; http://innovareacademics.in/journals/index.php/ijpps/article/view/2954

Objective: Mefenamic Acid (MA) is a non-steroidal anti-inflammatory drug (NSAIDs). This drug provides analgesic and antipyretic (fever reducing effect) and higher doses, anti-inflammatory effect. This study is focused to develop a rapid and sensitive method for the detection of mefenamic acid in human plasma. Methods: Protein precipitation technique using acetonitrile was used. Chromatographic separation was achieved on Agilent Zorbax Eclipse XDB-C18 Results: The recovery was found 83% for MA. The method was validated according to the Centre for Drug Evaluation and Research (CDER) guidelines. Calibration plot was linear within the range from 250 to 5000ng ml(150 mm x 4.6 mm, i. d 3.5 μm) with a mobile phase consisting of acetonitrile and 2% triethylamine (pH was adjusted to 4.2 with phosphoric acid) in a ratio of 60:40. The retention time for mefenamic acid and diclofenac was 5.4 and 3.9 minutes respectively. The mefenamic acid was monitored at 280 nm using variable-wavelength detector. -1 with the coefficient of determination (r2 Conclusion: The results were in compliance with CDER guideline. ) of ≥ 0.99. The quality control samples of mefenamic acid which was termed as low (L), medium (M) and high (H) were analysed to get the precision and accuracy. The accuracy for intra-day for L, M and H was 99.71%, 93.8% and 89.52% while for inter day were 97.67%, 93.46% and 91.67% respectively. On the other hand, coefficient variance (CV) for intra-day precision for L, M and H was found 2.57%, 2.45% and 1.45% and for inter day CV were 3.11%, 5.5% and 4.37% respectively. Diclofenac sodium was used as internal standard for this study. Keywords: HPLC, Mefenamic Acid, Human plasma, UV detector, Protein precipitation.