Starvation time and successive collection effects on leeches saliva collection quantity and proteins quality and quantity in wet season (Kesan masa kebuluran dan kejayaan pengumpulan kuantiti air liur lintah serta kualiti dan kuantiti protein dalam musim hujan)
The salivary gland secretion of the haematophagous animals, leeches, has attracted the attention of therapists since the extreme old ages due to its wide range of medical properties. Thus, many researches have been done to develop and optimize new methods to collect leech saliva with high quality...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit UKM
2014
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/40409/1/Starvation_Time_and_Successive_Collection_Effects_on_Leeches_Saliva_Collection_Quantity_and_Proteins_Quality_and_Quantity_in_Wet_Season.pdf http://irep.iium.edu.my/40409/ http://www.ukm.my/jsm/pdf_files/SM-PDF-43-11-2014/07%20Mohamed%20Alaama.pdf |
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| Summary: | The salivary gland secretion of the haematophagous animals, leeches, has attracted the attention of therapists since
the extreme old ages due to its wide range of medical properties. Thus, many researches have been done to develop and
optimize new methods to collect leech saliva with high quality and quantity. In the present study, we aimed to evaluate
the effects of starvation period and repeated collection on the quality and quantity of leech saliva extract
LSE
and its
contents of proteins during the rainy season. Protein recovery in the
LSE
was also studied after first collection. It was
found that leeches are able to produce protein-containing saliva whenever fed during the whole study period of 18 weeks
with varied protein concentrations. The results showed that the highest protein concentrations (105-91
μ
g/mL
) were
produced after 12-15 weeks of starvation. The results of successive collection showed that leeches are able to produce
proteins and peptides whenever they suck the solution after first collection with some varies in the concentrations. The
concentrations varied between 0 and 72% of the initial concentration. Gel electrophoresis results showed absence for
some bands when the concentrations are too low. Also the results showed that leeches are able to recover about 42%
of their initial proteins concentration within four weeks of starvation after first feeding. The gel electrophoresis results
showed the closeness between the first and second collections. To conclude, all test factors (starvation period, successive
collection and recovery test) were shown to have an important impact on protein concentration of leech saliva and therefore
its medicinal affectivity. The mentioned results are reported for the first time and they open the gate for further studies.
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