Quantification of nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy
Objective: Quantification of medicinal plant, N. sativa oil, in biodegradable nanoparticles fabricated from PLGA and chitosan was impossible due to the difficulty in the oil extraction-partition method of which the nanoparticle did not dissolve in any solvent. Methods: FTIR method was developed t...
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my.iium.irep.396442015-03-11T08:36:02Z http://irep.iium.edu.my/39644/ Quantification of nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy Doolaanea, Abd Almonem Harun Ismail, Ahmad Fahmi Mohamed, Farahidah QD Chemistry RS Pharmacy and materia medica Objective: Quantification of medicinal plant, N. sativa oil, in biodegradable nanoparticles fabricated from PLGA and chitosan was impossible due to the difficulty in the oil extraction-partition method of which the nanoparticle did not dissolve in any solvent. Methods: FTIR method was developed to quantify the loading efficiency of NSO from the intact nanoparticles without the need to solubilise the nanoparticles and extract the NSO thereafter. Beer-Lambert law was applied in the quantification following selection of a few wave number combinations. Results: The method exhibited linearity in the range NSO/PLGA=5-150% with R2=0.9911, RSD=1.68%, LOD=0.89% and LOQ=2.68%. NSO-PLGA nanoparticles revealed complete encapsulation of NSO (loading efficiency=101.5±2.2%) while chitosan-NSO-PLGA nanoparticle showed lower loading efficiency (84.5±1.7%) due to presence of the hydrophilic polymer, i. e. chitosan. This method is fast and easy to apply and does not require sample processing. The method will help to accelerate and improve routine characterisation of NSO nanoparticles during development and optimisation stage. Academic Sciences 2014 Article REM application/pdf en http://irep.iium.edu.my/39644/1/Quantification_of_Nigella_Sativa_Oil_%28NSO%29_from_Biodegradable_PLGA_Nanoparticles_Using_FTIR_Spectroscopy.pdf Doolaanea, Abd Almonem and Harun Ismail, Ahmad Fahmi and Mohamed, Farahidah (2014) Quantification of nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy. International Journal of Pharmacy and Pharmaceutical Sciences, 6 (10). pp. 228-232. ISSN 0975-1491 http://innovareacademics.in/journals/index.php/ijpps/article/view/2529 |
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QD Chemistry RS Pharmacy and materia medica Doolaanea, Abd Almonem Harun Ismail, Ahmad Fahmi Mohamed, Farahidah Quantification of nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy |
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Objective: Quantification of medicinal plant, N. sativa oil, in biodegradable nanoparticles fabricated from PLGA and chitosan was impossible due to the difficulty in the oil extraction-partition method of which the nanoparticle did not dissolve in any solvent.
Methods: FTIR method was developed to quantify the loading efficiency of NSO from the intact nanoparticles without the need to solubilise the nanoparticles and extract the NSO thereafter. Beer-Lambert law was applied in the quantification following selection of a few wave number combinations.
Results: The method exhibited linearity in the range NSO/PLGA=5-150% with R2=0.9911, RSD=1.68%, LOD=0.89% and LOQ=2.68%. NSO-PLGA nanoparticles revealed complete encapsulation of NSO (loading efficiency=101.5±2.2%) while chitosan-NSO-PLGA nanoparticle showed lower loading efficiency (84.5±1.7%) due to presence of the hydrophilic polymer, i. e. chitosan. This method is fast and easy to apply and does not require sample processing. The method will help to accelerate and improve routine characterisation of NSO nanoparticles during development and optimisation stage. |
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Article |
author |
Doolaanea, Abd Almonem Harun Ismail, Ahmad Fahmi Mohamed, Farahidah |
author_facet |
Doolaanea, Abd Almonem Harun Ismail, Ahmad Fahmi Mohamed, Farahidah |
author_sort |
Doolaanea, Abd Almonem |
title |
Quantification of nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy |
title_short |
Quantification of nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy |
title_full |
Quantification of nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy |
title_fullStr |
Quantification of nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy |
title_full_unstemmed |
Quantification of nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy |
title_sort |
quantification of nigella sativa oil (nso) from biodegradable plga nanoparticles using ftir spectroscopy |
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Academic Sciences |
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2014 |
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http://irep.iium.edu.my/39644/1/Quantification_of_Nigella_Sativa_Oil_%28NSO%29_from_Biodegradable_PLGA_Nanoparticles_Using_FTIR_Spectroscopy.pdf http://irep.iium.edu.my/39644/ http://innovareacademics.in/journals/index.php/ijpps/article/view/2529 |
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