Screening of important autoinduction medium composition for high biaomass production of E. coli expressing recombinant bromelain
Bromelain, a naturally available therapeutic protease enzyme from pineapple stem, was expressed in Escherichia coli using autoinduction-based fermentation medium. There are numerous recombinant proteins functionally expressed in E. coli using this autoinduction approach. Preliminary media trials...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Journal of Pure and Applied Microbiology
2014
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/38448/1/May_Jamil_JPAM.pdf http://irep.iium.edu.my/38448/3/38448_Screening%20of%20important_scopus.pdf http://irep.iium.edu.my/38448/ http://www.microbiologyjournal.org/ |
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| Summary: | Bromelain, a naturally available therapeutic protease enzyme from pineapple
stem, was expressed in Escherichia coli using autoinduction-based fermentation medium.
There are numerous recombinant proteins functionally expressed in E. coli using this
autoinduction approach. Preliminary media trials have shown that this medium is
capable of producing a high cell density batch culture as claimed. Since the level of
soluble expression is both protein and host/strain specific, further screening to identify
significant media components affecting biomass production is therefore required.
Currently, screening of full autoinduction medium components has yet to be examined
elsewhere in the literature due to its tedious nature. Hence, the application of a fractional
factorial design for identifying significant components in the autoinduction formulation
was reported. Statistical analysis showed that glucose, glycerol and L-arabinose were
most significant components influencing the biomass production. However, their effects
were demoting rather than promoting the biomass production at elevated concentrations
in shake flask culture. The highest biomass production (7.3 g/l) was achieved at low
levels (-1). This represents 0.05 % (w/v) glucose, 0.5 % (w/v) glycerol, without the need of
additional L-arabinose inducer. For a low number of experimental runs, this statistical
approach has been proven efficient for screening vital medium components in comparison
to conventional method. |
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