Statistical optimization of bioethanol production from empty fruit bunches by a mixed culture of yeast and fungus
Production of bioethanol from oil palm empty fruit bunches (EFB) by Saccharomyces cerevisae and Aspergillus niger is among the ways of reducing environmental pollution and consumption of crude oil. This study used sequential optimization approach based on statistical experimental design includ...
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| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2014
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/34996/1/ICBioE-14-hidayah.PDF http://irep.iium.edu.my/34996/ |
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| Summary: | Production of bioethanol from oil palm empty fruit bunches (EFB) by Saccharomyces cerevisae and
Aspergillus niger is among the ways of reducing environmental pollution and consumption of crude
oil. This study used sequential optimization approach based on statistical experimental design
including Plackett-Burman (PB) design, one-factor-at-a-time (OFAT) and face-centered central
composite design (FCCCD). Plackett-Burman design was used to screen the important medium
components and process conditions that influence the bioethanol production. The three parameters
chosen based on the PB analysis for determination of optimum values by response surface
methodology (RSM) were pH, KH2PO4 and agitation. The validity of the model was verified and
the optimum value of pH 5.5, agitation of 150rpm and 0.3% of KH2PO4 led to a maximum
bioethanol production of 7.4 g/l. |
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