Increased of Bcl-2/Bax ratio corelated with decreased of lymphocyte apoptosis: a study in the bronchiolus and lung of asthmatic mice
The failure of lymphocyte apoptosis is one of the factors causing chronic airway inflammation in asthma. Some studies indicate the role of Bcl-2 in inhibition of lymphocyte apoptosis, but still little research is on the role of Bax and its relationship to Bcl-2 in asthma. The purpose of this study...
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| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/33117/1/taufiq_hidayat.pdf http://irep.iium.edu.my/33117/ |
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| Summary: | The failure of lymphocyte apoptosis is one of the factors causing chronic airway inflammation in
asthma. Some studies indicate the role of Bcl-2 in inhibition of lymphocyte apoptosis, but still little
research is on the role of Bax and its relationship to Bcl-2 in asthma. The purpose of this study was to
prove the role of Bcl-2-lymphocyte in inhibition of lymphocyte apoptosis and decrease in Baxlymphocytes
in bronchiolus and lung of asthmatic mice. This study was a randomized control group
design. Subjects were Balb/c mice which divided into 2 groups: non-asthma and asthma. Asthma group
were sensitized with ovalbumin intraperitoneally on day 0 and 14, followed by inhalation every 2 to 3
days for 6 weeks. At week 8, all subjects terminated. Bcl-2 and Bax-lymphocytes expression were
examined with immunohistochemical method, whereas apoptotic lymphocytes by Terminal
deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method. Statistical analysis used was the
independent sample t-test and regression analysis, with 95% confidence interval. Bronchial and lung
specimens were obtained from 18 subjects (9 from each group). The ratio of apoptotic lymphocytes
decreased in the asthma group (p = 0.003), Bcl-2-lymphocytes increased in the asthma group (p <
0.001), and Bax-lymphocytes decreased in the asthma group (p = 0.003). There was a strong negative
correlation (r = -0.66, r2 = 0.43, p = 0.003) between the Bcl-2 and lymphocyte apoptosis. There was also a
strong negative correlation (r = -0.56, r2 = 0.35, p = 0.009) between the ratio Bcl-2/Bax and lymphocyte
apoptosis. However, no significant relationship was found between Bax-lymphocytes and lymphocyte
apoptosis (r = 0.36, r2 = 0.13, p = 0.15). Increasing the ratio of Bcl-2/Bax inhibit lymphocyte apoptosis
where Bcl-2 plays more role than Bax. |
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