Purification of antibacterial compounds from spathiphyllum cannifolium leaf
Spathiphyllum cannifolium is an ‘araceae’ species which can be found in Malaysia for landscaping. Previous study carried out in this lab found that this plant possesses the highest antibacterial activity among 19 flowering plants screened. In the present study, purification of antibacterial compou...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/29936/1/paper_Der_Pharma_Chemica.pdf http://irep.iium.edu.my/29936/ |
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| Summary: | Spathiphyllum cannifolium is an ‘araceae’ species which can be found in Malaysia for landscaping. Previous study
carried out in this lab found that this plant possesses the highest antibacterial activity among 19 flowering plants
screened. In the present study, purification of antibacterial compound of the plant was conducted. For purification
of antibacterial compound, open column chromatography was conducted and ethyl acetate, hexane and ethanol are
used as the mobile phase. The results showed that the elution of column with 80% ethyl acetate in ethanol provide
fractions with the highest antibacterial activity. The fractions were analyzed with TLC plates and revealed a single spot under UV light at 0.85 Rf value. Further purification of the single spot using HPLC showed two isolated compounds at retention time of 5.53 minutes and 8.26 minutes were obtained. The compounds were suspected as
flavanoids as they can be detected at wavelength 360 nm and 400 nm. |
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