Analysis of fucoxanthin content and purification of all-trans-fucoxanthin from turbinaria turbinata and sargassum plagyophyllum by SiO2 open column chromatography and reversed phase-HPLC

• Main Authors: Jaswir, Irwandi, Noviendri, Dedi, Mohd. Salleh, Hamzah, Bakhtiar, M. Taher, Miyashita, Kazuo, M. Ramli, Nazaruddin
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2013
• Subjects: QD Chemistry; TP248.13 Biotechnology
• Online Access: http://irep.iium.edu.my/28716/1/JLRCT-2013.pdf; http://irep.iium.edu.my/28716/; http://www.tandfonline.com/doi/abs/10.1080/10826076.2012.691435#preview

Reversed phase-high performance liquid chromatography (RP-HPLC) was used to analyze fucoxanthin content of two species of Malaysian brown seaweeds, Turbinaria turbinate andSargassum plagyophyllum. The fucoxanthin contents of T. turbinata, and S. plagyophyllum were 0.59 ± 0.08 and 0.71 ± 0.01 mg/g dry-weight, respectively. Ultraviolet spectrum of fucoxanthin showed the maximum absorbance at 450 nm. Furthermore, the HPLC chromatograms of purified all-trans-fucoxanthin from showed one major peak with a retention time of 7.8 minutes.