Supercritical Carbon Dioxide Extraction of Seed Oil from Winter Melon (Benincasa hispida) and Its Antioxidant Activity and Fatty Acid Composition
In the present study, supercritical carbon dioxide (SC-CO2) extraction of seed oil from winter melon (Benincasa hispida) was investigated. The effects of process variables namely pressure (150–300 bar), temperature (40–50 °C) and dynamic extraction time (60–120 min) on crude extraction yield (CEY) w...
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my.iium.irep.286312016-02-24T10:30:54Z http://irep.iium.edu.my/28631/ Supercritical Carbon Dioxide Extraction of Seed Oil from Winter Melon (Benincasa hispida) and Its Antioxidant Activity and Fatty Acid Composition Bimakr, Mandana Rahman, Russly Taip, Farah Saleena Mohd Adzahan, Noranizan Sarker, Md. Zaidul Islam Ganjloo, Ali QD Chemistry In the present study, supercritical carbon dioxide (SC-CO2) extraction of seed oil from winter melon (Benincasa hispida) was investigated. The effects of process variables namely pressure (150–300 bar), temperature (40–50 °C) and dynamic extraction time (60–120 min) on crude extraction yield (CEY) were studied through response surface methodology (RSM). The SC-CO2 extraction process was modified using ethanol (99.9%)as co-solvent. Perturbation plot revealed the significant effect of all process variables on the CEY. A central composite design (CCD) was used to optimize the process conditions to achieve maximum CEY. The optimum conditions were 244 bar pressure, 46 °C temperature and 97 min dynamic extraction time. Under these optimal conditions, the CEY was predicted to be 176.30 mg-extract/g-dried sample. The validation experiment results agreed with the predicted value. The antioxidant activity and fatty acid composition of crude oil obtained under optimized conditions were determined and compared with published results using Soxhlet extraction (SE) and ultrasound assisted extraction (UAE). It was found that the antioxidant activity of the extract obtained by SC-CO2 extraction was strongly higher than those obtained by SE and UAE. Identification of fatty acid composition using gas chromatography (GC) showed that all the extracts were rich in unsaturated fatty acids with the most being linoleic acid. In contrast, the amount of saturated fatty acids extracted by SE was higher than that extracted under optimized SC-CO2 extraction conditions. MDPI 2013-01-15 Article REM application/pdf en http://irep.iium.edu.my/28631/2/Molecules-Zaidul-Mandana.pdf Bimakr, Mandana and Rahman, Russly and Taip, Farah Saleena and Mohd Adzahan, Noranizan and Sarker, Md. Zaidul Islam and Ganjloo, Ali (2013) Supercritical Carbon Dioxide Extraction of Seed Oil from Winter Melon (Benincasa hispida) and Its Antioxidant Activity and Fatty Acid Composition. Molecules, 18 (1). pp. 997-1014. ISSN 1420-3049 http://www.mdpi.com/journal/molecules doi:10.3390/molecules18010997 |
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QD Chemistry Bimakr, Mandana Rahman, Russly Taip, Farah Saleena Mohd Adzahan, Noranizan Sarker, Md. Zaidul Islam Ganjloo, Ali Supercritical Carbon Dioxide Extraction of Seed Oil from Winter Melon (Benincasa hispida) and Its Antioxidant Activity and Fatty Acid Composition |
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In the present study, supercritical carbon dioxide (SC-CO2) extraction of seed oil from winter melon (Benincasa hispida) was investigated. The effects of process variables namely pressure (150–300 bar), temperature (40–50 °C) and dynamic extraction time (60–120 min) on crude extraction yield (CEY) were studied through response surface
methodology (RSM). The SC-CO2 extraction process was modified using ethanol (99.9%)as co-solvent. Perturbation plot revealed the significant effect of all process variables on the CEY. A central composite design (CCD) was used to optimize the process conditions to
achieve maximum CEY. The optimum conditions were 244 bar pressure, 46 °C temperature and 97 min dynamic extraction time. Under these optimal conditions, the CEY was predicted
to be 176.30 mg-extract/g-dried sample. The validation experiment results agreed with the predicted value. The antioxidant activity and fatty acid composition of crude oil obtained under optimized conditions were determined and compared with published results using Soxhlet extraction (SE) and ultrasound assisted extraction (UAE). It was found that the antioxidant activity of the extract obtained by SC-CO2 extraction was strongly higher than those obtained by SE and UAE. Identification of fatty acid composition using gas chromatography (GC) showed that all the extracts were rich in unsaturated fatty acids with the most being linoleic acid. In contrast, the amount of saturated fatty acids extracted by SE was higher than that extracted under optimized SC-CO2 extraction conditions. |
format |
Article |
author |
Bimakr, Mandana Rahman, Russly Taip, Farah Saleena Mohd Adzahan, Noranizan Sarker, Md. Zaidul Islam Ganjloo, Ali |
author_facet |
Bimakr, Mandana Rahman, Russly Taip, Farah Saleena Mohd Adzahan, Noranizan Sarker, Md. Zaidul Islam Ganjloo, Ali |
author_sort |
Bimakr, Mandana |
title |
Supercritical Carbon Dioxide Extraction of Seed Oil from
Winter Melon (Benincasa hispida) and Its Antioxidant Activity and Fatty Acid Composition |
title_short |
Supercritical Carbon Dioxide Extraction of Seed Oil from
Winter Melon (Benincasa hispida) and Its Antioxidant Activity and Fatty Acid Composition |
title_full |
Supercritical Carbon Dioxide Extraction of Seed Oil from
Winter Melon (Benincasa hispida) and Its Antioxidant Activity and Fatty Acid Composition |
title_fullStr |
Supercritical Carbon Dioxide Extraction of Seed Oil from
Winter Melon (Benincasa hispida) and Its Antioxidant Activity and Fatty Acid Composition |
title_full_unstemmed |
Supercritical Carbon Dioxide Extraction of Seed Oil from
Winter Melon (Benincasa hispida) and Its Antioxidant Activity and Fatty Acid Composition |
title_sort |
supercritical carbon dioxide extraction of seed oil from
winter melon (benincasa hispida) and its antioxidant activity and fatty acid composition |
publisher |
MDPI |
publishDate |
2013 |
url |
http://irep.iium.edu.my/28631/2/Molecules-Zaidul-Mandana.pdf http://irep.iium.edu.my/28631/ http://www.mdpi.com/journal/molecules |
_version_ |
1643609532141666304 |
score |
13.18916 |