Study on antidiabetic plants cocktail containing Andrographis paniculata, Cinnamomum burmannii and Tinospora crispa

• Main Authors: Bakhtiar, M. Taher, Mohamed Amiroudine, Mohamed Zaffar Ali, Tg Zakaria, Tg Muhammad Faris Syafiq, Wan Sulaiman, Wan Mohd. Azizi, Darnis, Deny Susanti, Arief Ichwan, Solachuddin Jauhari
• Format: Article
• Language: English
• Published: Penerbit USM 2012
• Subjects: RS Pharmacy and materia medica
• Online Access: http://irep.iium.edu.my/26473/1/Malaysian_Journal_of_Pharmacy.pdf; http://irep.iium.edu.my/26473/; http://www.mps.org.my/index.cfm?&menuid=146

Andrographis paniculata, Cinnamomum burmannii and Tinospora crispa (ACT) have been shown to have antidiabetes activity. The present study was aimed to investigate the antidiabetic potential of the mixture of ACT in vivo and in vitro. A set dose of ACT (500, 125 and 75 mg/kg b.w. respectively) were administered to both normal and streptozotocin induced diabetic male Sprague-Dawley rats. The blood glucose levels were measured at 0, 3, 7, and 10 days after oral administration of ACT. Comparison was made with glibenclamide and placebo group. In in vitro method, the effect of ACT on lipid accumulation of 3T3-L1 adipocytes was analyzed by using Oil Red O staining. 2-Deoxy-D-[3H] glucose was used to measure glucose uptake activity. As the results, ACT exhibited blood lowering activity in streptozotocin induced diabetic rats. In cell based study, ACT was found to induce triglyceride accumulation in 3T3-L1 cells at relatively small concentration in a dose-dependent manner. In addition, 2-deoxy-D-[3H] glucose uptake activities were significantly different at dose 50 µg/mL (P < 0.05 compared to basal). Gene expression analysis using quantitative RT-PCR showed that adipocytes-treated with ACT significantly increased the expression levels of PPARγ which regulate the adipogenesis process and its target gene GLUT4 that involve in glucose uptake when compared to the basal. Taken together, the results of this experimental study indicate that ACT possesses antihyperglycemic effects in vivo and in vitro.