Molecular cloning of phytase gene from ASUIA279 and its expression in Pichia Pastoris system

• Main Authors: Maifiah, M.H. Mahamad, Jamal, Parveen, Nuge, Tamrin, Mohd Dali, Nor Soleha, Meor Hussin, Anis Shobirin, Farouk, Abd Alaziem, Mohd. Salleh, Hamzah
• Format: Article
• Language: English
• Published: IIUM Press 2011
• Subjects: QH426 Genetics
• Online Access: http://irep.iium.edu.my/17046/4/219-1318-1-PB.pdf; http://irep.iium.edu.my/17046/; http://www.iium.edu.my/ejournal/index.php/iiumej/article/view/211

Phytase gene obtained from Bacillus subtilis ASUIA243 was cloned into a medium vector and transformed into E. coli. Restriction enzyme digestion was conducted to get blunt-ended phytase gene and ligated into the Pichia expression vector, pPICZαA. The recombinant vector, pPICZαA-243HPp was then li...