Isolation of fuxoxanthin and fatty analysis from Padina australis and cytotoxicity effect of fucoxanthin of human lung cancer (H1299) cell lines
Fucoxanthin has been successfully isolated from species of Malaysian brown seaweed, namely P. australis. The purity of the fucoxanthin is >98% as indicated by High Performance Liquid Chromatography analysis. This seaweed also contained a considerable amount of unsaturated fatty acids. There are t...
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| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/16360/1/Isolation_of_Fucoxanthin_and_fatty_acids_analysis.pdf http://irep.iium.edu.my/16360/ http://www.iium.edu.my/icbioe/2011/ |
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| Summary: | Fucoxanthin has been successfully isolated from species of Malaysian brown seaweed, namely P. australis. The purity of the fucoxanthin is >98% as indicated by High Performance Liquid Chromatography analysis. This seaweed also contained a considerable amount of unsaturated fatty acids. There are thirteen fatty acids were detected with Gas Chromatography. However, in terms of fatty acid methyl ester (FAMEs) of eicosapentanoic acid (C20:5n-3), arachidonic acid (C20:4n-6), linoleic acid (C18:2n-6) and alpha-linolenic acid (C18:3n-3) contents from P.australis was found (2.06, 9.50, 6.37, and 2.83 %, respectively). For saturated fatty acids, palmitic acid (C16:0) was found to be the major fatty acid about 23.97%. Furthermore, data obtained from the MTT assay indicated that fucoxanthin reduced the viability of H1299 cell lines, showing an IC50 value was 2.45 mM. |
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