Evaluation of nutritional components by Plackett-Burman design for Penicillium citrinum lipase production using palm oil mill effluent

A number of medium components influencing lipase production by Penicillium citrinum (ATCC 42799) were studied using palm oil mill effluent (POME) as the basal medium. The medium components (peptone, yeast extract, malt extract, NH4Cl, NaNO3, KH2PO4, CaCl2, MgSO4, olive oil and Tween-80) were anal...

Full description

Saved in:
Bibliographic Details
Main Authors: Salihu, Aliyu, Alam, Md. Zahangir, Abdulkarim, Mohd Ismail, Hamzah, Mohd. Salleh
Format: Article
Language:English
Published: Academic Journals 2011
Subjects:
Online Access:http://irep.iium.edu.my/14377/1/AJB-Aliyu.pdf
http://irep.iium.edu.my/14377/
http://www.academicjournals.org/AJB/abstracts/abs2011/16DecConf/Salihu%20et%20al.htm
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A number of medium components influencing lipase production by Penicillium citrinum (ATCC 42799) were studied using palm oil mill effluent (POME) as the basal medium. The medium components (peptone, yeast extract, malt extract, NH4Cl, NaNO3, KH2PO4, CaCl2, MgSO4, olive oil and Tween-80) were analyzed in twelve experimental trials using Plackett–Burman (PB) design. The most significant components affecting lipase production were found to be Tween-80, peptone, yeast extract, malt extract and NaNO3 at p < 0.05. The results indicate the efficiency of using PB design for screening processes. However, optimal concentration of the significant components can be determined by further statistical analysis.