Fabrication of ceramic membrane chromatography for biologics purification
Chromatography is one of the most important separation processes of choice for the recovery/purification of proteins and complex bio-structures. Fabrication of chromatographic membranes and their efficiency in the chromatography process has been the subject of many recent researches. In this stud...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
IIUM Press
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/13933/1/209_Ceramic_membrane.pdf http://irep.iium.edu.my/13933/ http://www.iium.edu.my/ejournal/index.php/iiumej/article/viewArticle/213 |
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| Summary: | Chromatography is one of the most important separation processes of
choice for the recovery/purification of proteins and complex bio-structures. Fabrication
of chromatographic membranes and their efficiency in the chromatography process has
been the subject of many recent researches. In this study, a coin-like, 13 mm diameter
and 3 mm thick, ceramic membrane was fabricated to be used as a chromatographic
medium. The membrane is used to replace the conventional resin-based chromatography
columns. Hydroxyapatite (HA) powder was used as a material for the membrane
fabrication. In this project, a HA powder was produced using starch as pore creating
agents. Characterization processes were done for the ceramic membrane using the
suitable apparatuses. Three parameters of the fabrication process (starch wt %,
compaction pressure and sintering temperature) were manipulated to optimize the
performance of the membrane. The fabricated membrane was placed in a (FPLC)
system to be tested for its performance as an adsorptive membrane. (IMAC) process was
run by immobilizing Ni2+ ions at the membrane particles surfaces. NP protein of the
(NDV) was used to test the membrane’s ability to bind Histidine-tagged proteins. The
optimum set of process parameters that yielded in the highest porosity and good
chromatogram was determined to be 5 wt % starch, 3000 psi compaction pressure and
1100°C sintering temperature. |
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