Optimization of Newcastle disease virus production in T-flask

• Main Authors: Arifin, Mohd Azmir, Mel, Maizirwan, Salim, Siti Hajar, Abdul Karim, Mohd Ismail, Hassan, Sharifah Syed
• Format: Article
• Language: English
• Published: Academic Journals 2011
• Subjects: TP155 Chemical engineering; TP248.13 Biotechnology
• Online Access: http://irep.iium.edu.my/13687/1/Arifin_et_al.pdf; http://irep.iium.edu.my/13687/; http://www.academicjournals.org/AJB/abstracts/abs2011/16DecConf/Arifin%20et%20al.htm

Newcastle disease (ND) is regarded as one of the most important diseases in the poultry industry. Currently, vaccines for ND are produced by using embryonated chicken eggs, a method which has the disadvantages of being labour-intensive, time consuming and requires large area for the incubation of eggs. In the present study, the production of lentogenic Asplin F strain of Newcastle disease virus by using cell culture method was studied. Experiments were carried out in T-flasks to investigate the effects of serum concentration in the culture medium during virus replication phase and multiplicity of infection (MOI) on ND virus propagation in DF-1 cells. Results show that virus infectivity titre of 6.62 x 107 TCID50/ml was achieved when serum concentration of 0.5% and MOI of 20 was used