Evaluation of mutation effects on UGT1A1 activity toward 17beta-estradiol using liquid chromatography-tandem mass spectrometry

• Main Authors: Wada, Keiko, Takeuchi, Atsuko, Saiki, Kayoko, Sutomo, Retno, Van Rostenberghe, Hans, Yusoff, Narazah Mohd, Laosombat, Vichai, Sadewa, Ahmad Hamim, A.Talib, Norlelawati, Yusoff, Surini, Lee, Myeong Jin, Ayaki, Hitoshi, Nakamura, Hajime, Matsuo, Masafumi, Nishio, Hisahide
• Format: Article
• Language: English
• Published: 2006
• Subjects: R Medicine (General)
• Online Access: http://irep.iium.edu.my/12678/1/Wada_%2C_chromatography.pdf; http://irep.iium.edu.my/12678/; http://www.lib.kobe-u.ac.jp/handle_kernel/90001403

Mutations in the gene encoding UDP-glucuronosyltransferase 1A1 (UGT1A1) may reduce the glucuronidation of estradiol, bilirubin, etc. In the present study, we used a liquid chromatography-tandem mass spectrometry (LC/MS/MS) method to assay the activities of recombinant mutated UGT1A1 toward 17beta-es...