Isolation and characterization of ssDNA aptamers against BipD antigen of Burkholderia pseudomallei

• Main Authors: Selvam, Kasturi, Najib, Mohamad Ahmad, Khalid, Muhammad Fazli, Yunus, Muhammad Hafiznur, A. Wahab, Habibah, Harun, Azian, Zainulabid, Ummu Afeera, Mustaffa, Khairul Mohd Fadzli, Ismail, Aziah
• Format: Article
• Language: English
• Published: Academic Press Inc. 2024
• Subjects: RC111 Infectious and Parasitic Diseases
• Online Access: http://irep.iium.edu.my/115497/7/115497_Isolation%20and%20characterization%20of%20ssDNA%20aptamers%20against%20BipD.pdf; http://irep.iium.edu.my/115497/; https://sciencedirect.com/science/article/abs/pii/S0003269724001994#preview-section-introduction

Melioidosis is difficult to diagnose due to its wide range of clinical symptoms. The culture method is time-consuming and less sensitive, emphasizing the importance of rapid and accurate diagnostic tests for melioidosis. Burkholderia invasion protein D (BipD) of Burkholderia pseudomallei is a potential diagnostic biomarker. This study aimed to isolate and characterize single-stranded DNA aptamers that specifically target BipD.