Bacterial cellulose production from oil palm frond juice and its impregnation with silver nanoparticles for antibacterial wound dressing

• Main Authors: Tan, Sou Min, Abd Karim, Nor Syahirah, Mohamad, Shahril, Zakaria, Junaidi, Arzmi, Mohd Hafiz, Ismail, Hassan Fahmi, Abu, Rohana
• Format: Article
• Language: English
• Published: Malaysian Palm Oil Board 2023
• Subjects: QR Microbiology
• Online Access: http://irep.iium.edu.my/109439/7/109439_Bacterial%20cellulose%20production%20from%20oil%20palm.pdf; http://irep.iium.edu.my/109439/; http://jopr.mpob.gov.my/bacterial-cellulose-production-from-oil-palm-frond-juice-and-its-impregnation-with-silver-nanoparticles-for-antibacterial-wound-dressing/

Bacterial cellulose-silver nanoparticles (BC-AgNPs) composite was prepared for a bacterial wound dressing. The oil palm frond (OPF) juice was utilized as a low-cost raw material for BC production. The AgNPs were incorporated in the BC composite by thermal reduction of 1 mM silver nitrate for their antibacterial agent. The BC-AgNPs composite had dense nanofibrils with an average diameter of 61.5±1.0 nm as shown in the field emission scanning electron microscope (FESEM) images. The composite had a crystallinity index of 86.5% and the nanoparticles had a face-centred cubic geometry with a crystal size of 26.5 nm as determined by x-ray diffraction analysis (XRD). There was 1.463 mg/100 cm2 of Ag in the composite analysed by atomic absorption spectrophotometry (AAS). 10.4% of the total Ag content was released from AgNPs in 72 h as measured by inductively coupled plasma mass spectrometry (ICP-MS). The composite also demonstrated excellent antibacterial action against Staphylococcus aureus, giving a 29±0.8 mm inhibition zone by the disk diffusion assay. Pure BC composite exhibited no cytotoxicity effect on the HSF1184 fibroblast cells and 10-40 % BC-AgNPs composite extracts was compatible with the cell growth. The study suggests the BC-AgNPs composite is a good material for antibacterial wound dressing.