Validation of IVCM in measuring sub-basal nerve plexus and keratocyte cell density in corneal wound healing
Introduction: This study aimed to evaluate the reliability measurement of corneal stromal thickness, sub-basal nerve plexus (SBNP) and keratocyte cell density (KCD) in laser refractive surgery patients. Methods: 120 eyes of 60 participants were recruited and both right and left eyes of the myopic...
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my.iium.irep.1089992023-12-20T01:05:58Z http://irep.iium.edu.my/108999/ Validation of IVCM in measuring sub-basal nerve plexus and keratocyte cell density in corneal wound healing Md Rejab, Noor Shazana Hilmi, Mohd Radzi Mohd. Kamal, Khairidzan RE Ophthalmology Introduction: This study aimed to evaluate the reliability measurement of corneal stromal thickness, sub-basal nerve plexus (SBNP) and keratocyte cell density (KCD) in laser refractive surgery patients. Methods: 120 eyes of 60 participants were recruited and both right and left eyes of the myopic subjects were measured separately. Cornea stromal thickness were measured based on the cellular morphology that differs between each corneal layer. Measurement of SBNP and KCD were done using in-vivo confocal microscopy (IVCM) using corneal stromal thickness as reference. Corneal nerve parameters measured includes nerve fiber density (NFD), nerve branch Density (NBD) and nerve fiber length (NFL) while KCD were measured based the amount per area, depending on the region of interest. All images were captured and processed using ImageJTM Software and NeuronJ. All data were expressed in mean and standard deviation. Statistical analyses were performed using Predictive analytics software. P < 0.05 was set as the level of significance. Intra- and inter-observer intraclass correlation analysis were done to evaluate reliability of measurement in corneal stromal thickness, SBNP and KCD. Results: This study found no significant difference between measurements for corneal stromal thickness, SBNP and KCD measured. (All P > 0.05). Intraclass correlation analysis showed both intra- and inter-observer performance were approximately consistent and reliable (All r > 0.90, P > 0.05). Conclusion: Measurement of corneal stromal thickness, SBNP and KCD using IVCM is valid and reliable. Acquire Publications 2022-03-20 Article PeerReviewed application/pdf en http://irep.iium.edu.my/108999/13/108999_Validation%20of%20IVCM%20in%20measuring%20sub-basal%20nerve%20plexus.pdf Md Rejab, Noor Shazana and Hilmi, Mohd Radzi and Mohd. Kamal, Khairidzan (2022) Validation of IVCM in measuring sub-basal nerve plexus and keratocyte cell density in corneal wound healing. Journal of Ophthalmic Research and Vision Care, 2 (1). pp. 1-9. ISSN 2831-7459 https://www.acquirepublications.org/Journal/Ophthalmic/Articles/JORVC2200105 10.54289/JORVC2200105 |
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RE Ophthalmology Md Rejab, Noor Shazana Hilmi, Mohd Radzi Mohd. Kamal, Khairidzan Validation of IVCM in measuring sub-basal nerve plexus and keratocyte cell density in corneal wound healing |
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Introduction: This study aimed to evaluate the reliability measurement of corneal stromal thickness, sub-basal nerve plexus (SBNP) and keratocyte cell density (KCD) in laser refractive surgery patients.
Methods: 120 eyes of 60 participants were recruited and both right and left eyes of the myopic subjects were measured separately. Cornea stromal thickness were measured based on the cellular morphology that differs between each corneal layer. Measurement of SBNP and KCD were done using in-vivo confocal microscopy (IVCM) using corneal stromal thickness as reference. Corneal nerve parameters measured includes nerve fiber density (NFD), nerve branch Density (NBD) and nerve fiber length (NFL) while KCD were measured based the amount per area, depending on the region of interest. All images were captured and processed using ImageJTM Software and NeuronJ. All data were expressed in mean and standard deviation. Statistical analyses were performed using Predictive analytics software. P < 0.05 was set as the level of significance. Intra- and inter-observer intraclass correlation analysis were done to evaluate reliability of measurement in corneal stromal thickness, SBNP and KCD.
Results: This study found no significant difference between measurements for corneal stromal thickness, SBNP and KCD measured. (All P > 0.05). Intraclass correlation analysis showed both intra- and inter-observer performance were approximately consistent and reliable (All r > 0.90, P > 0.05).
Conclusion: Measurement of corneal stromal thickness, SBNP and KCD using IVCM is valid and reliable. |
format |
Article |
author |
Md Rejab, Noor Shazana Hilmi, Mohd Radzi Mohd. Kamal, Khairidzan |
author_facet |
Md Rejab, Noor Shazana Hilmi, Mohd Radzi Mohd. Kamal, Khairidzan |
author_sort |
Md Rejab, Noor Shazana |
title |
Validation of IVCM in measuring sub-basal nerve plexus and keratocyte cell density in corneal wound healing |
title_short |
Validation of IVCM in measuring sub-basal nerve plexus and keratocyte cell density in corneal wound healing |
title_full |
Validation of IVCM in measuring sub-basal nerve plexus and keratocyte cell density in corneal wound healing |
title_fullStr |
Validation of IVCM in measuring sub-basal nerve plexus and keratocyte cell density in corneal wound healing |
title_full_unstemmed |
Validation of IVCM in measuring sub-basal nerve plexus and keratocyte cell density in corneal wound healing |
title_sort |
validation of ivcm in measuring sub-basal nerve plexus and keratocyte cell density in corneal wound healing |
publisher |
Acquire Publications |
publishDate |
2022 |
url |
http://irep.iium.edu.my/108999/13/108999_Validation%20of%20IVCM%20in%20measuring%20sub-basal%20nerve%20plexus.pdf http://irep.iium.edu.my/108999/ https://www.acquirepublications.org/Journal/Ophthalmic/Articles/JORVC2200105 |
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1787131914664017920 |
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