Modeling of E. coli growth, glucose consumption, and recombinant collagen like protein formation kinetics
Mathematical modeling of the kinetics of fermentation processes is crucial to understand complex microorganism behavior and identify problems. To investigate growth kinetics, as well as glucose utilization and recombinant collagen-like protein formation kinetics, E. coli BL21 (DE3) harboring a colla...
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Kulliiyyah of Engineering, IIUM
2023
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my.iium.irep.1082612023-11-21T01:27:12Z http://irep.iium.edu.my/108261/ Modeling of E. coli growth, glucose consumption, and recombinant collagen like protein formation kinetics Mohamed Gameil, Abeir Hussein Yusof, Faridah Azmi, Azlin Suhaida Mohamad Puad, Noor Illi TP155 Chemical engineering TP248.13 Biotechnology Mathematical modeling of the kinetics of fermentation processes is crucial to understand complex microorganism behavior and identify problems. To investigate growth kinetics, as well as glucose utilization and recombinant collagen-like protein formation kinetics, E. coli BL21 (DE3) harboring a collagen-like protein from Rhodopseduomonas palustris (RPCLP) was cultivated in M9-casamino acids medium containing glucose (10 g/L). OD600 and a glucose assay kit were used to monitor the biomass concentration and glucose depletion, respectively. The RPCLP concentration was measured via a fluorometric assay. Results show that the maximum biomass achieved was 8.25 g/L, whereas the maximum RPCLP yield was 2 mg/L. Growth data was then fitted into unstructured models-Monod model and logistic model. The Luedeking-Piret model was used for the substrate utilization and product formation data. Kinetic parameters were determined by nonlinear regression and ANOVA analyses were done to determine the significance of each fit. While the growth data fitted both the Monod and the logistic model well, with significant coefficients of determination (R 2 values 0.96 and 0.99, respectively), the Luedeking-Piret models did not represent the substrate utilization and product formation well (correlation coefficient= 0.7). The study suggests that the production is mixed-growth associated and modification of the model is required. Kulliiyyah of Engineering, IIUM 2023-10-12 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/108261/1/108261_Modeling%20of%20E.%20coli%20growth.pdf Mohamed Gameil, Abeir Hussein and Yusof, Faridah and Azmi, Azlin Suhaida and Mohamad Puad, Noor Illi (2023) Modeling of E. coli growth, glucose consumption, and recombinant collagen like protein formation kinetics. In: International Conference on Chemical Engineering & Sustainability 2023 (ICCHES 2023), 15th - 16th August 2023, Kuala Lumpur, Malaysia. https://journals.iium.edu.my/ejournal/index.php/proc/article/view/3011 10.31436/iiumecp.v1i1.3011 |
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TP155 Chemical engineering TP248.13 Biotechnology Mohamed Gameil, Abeir Hussein Yusof, Faridah Azmi, Azlin Suhaida Mohamad Puad, Noor Illi Modeling of E. coli growth, glucose consumption, and recombinant collagen like protein formation kinetics |
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Mathematical modeling of the kinetics of fermentation processes is crucial to understand complex microorganism behavior and identify problems. To investigate growth kinetics, as well as glucose utilization and recombinant collagen-like protein formation kinetics, E. coli BL21 (DE3) harboring a collagen-like protein from Rhodopseduomonas palustris (RPCLP) was cultivated in M9-casamino acids medium containing glucose (10 g/L). OD600 and a glucose assay kit were used to monitor the biomass concentration and glucose depletion, respectively. The RPCLP concentration was measured via a fluorometric assay. Results show that the maximum biomass achieved was 8.25 g/L, whereas the maximum RPCLP yield was 2 mg/L. Growth data was then fitted into unstructured models-Monod model and logistic model. The Luedeking-Piret model was used for the substrate utilization and product formation data. Kinetic parameters were determined by nonlinear regression and ANOVA analyses were done to determine the significance of each fit. While the growth data fitted both the Monod and the logistic model well, with significant coefficients of determination (R 2 values 0.96 and 0.99, respectively), the Luedeking-Piret models did not represent the substrate utilization and product formation well (correlation coefficient= 0.7). The study suggests that the production is mixed-growth associated and modification of the model is required. |
format |
Proceeding Paper |
author |
Mohamed Gameil, Abeir Hussein Yusof, Faridah Azmi, Azlin Suhaida Mohamad Puad, Noor Illi |
author_facet |
Mohamed Gameil, Abeir Hussein Yusof, Faridah Azmi, Azlin Suhaida Mohamad Puad, Noor Illi |
author_sort |
Mohamed Gameil, Abeir Hussein |
title |
Modeling of E. coli growth, glucose consumption, and recombinant collagen like protein formation kinetics |
title_short |
Modeling of E. coli growth, glucose consumption, and recombinant collagen like protein formation kinetics |
title_full |
Modeling of E. coli growth, glucose consumption, and recombinant collagen like protein formation kinetics |
title_fullStr |
Modeling of E. coli growth, glucose consumption, and recombinant collagen like protein formation kinetics |
title_full_unstemmed |
Modeling of E. coli growth, glucose consumption, and recombinant collagen like protein formation kinetics |
title_sort |
modeling of e. coli growth, glucose consumption, and recombinant collagen like protein formation kinetics |
publisher |
Kulliiyyah of Engineering, IIUM |
publishDate |
2023 |
url |
http://irep.iium.edu.my/108261/1/108261_Modeling%20of%20E.%20coli%20growth.pdf http://irep.iium.edu.my/108261/ https://journals.iium.edu.my/ejournal/index.php/proc/article/view/3011 |
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1783876126583554048 |
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13.160551 |