Tongkat ali root extract helps promote good oral health

• Main Authors: Ramzi, Muhamad Iyad, Kosnin, Muhammad Hazzim, Faisal, Ghasak Ghazi, Arzmi, Mohd Hafiz, Kusumawardani, Anisa
• Format: Proceeding Paper
• Language: English; English; English; English
• Published: 2023
• Subjects: RK Dentistry; RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works
• Online Access: http://irep.iium.edu.my/107931/1/GHASAK-IIUM-POSTER-WITHOUTVIDEO%20-%20Copy.pdf; http://irep.iium.edu.my/107931/3/NOHRI%20Book%20Final.pdf; http://irep.iium.edu.my/107931/2/E-Poster%20Presentation%20%28Senior%20Researcher%20Category%29-1-2.pdf; http://irep.iium.edu.my/107931/19/107931_Tongkat%20ali%20root%20extract%20helps%20promote%20good%20oral%20health.pdf; http://irep.iium.edu.my/107931/; https://conference.iium.edu.my/nohri2023/index.php/about-nohri-2/

Introduction: One of the important issues in oral healthcare is prevention of dental caries through maintenance of good oral hygiene. Despite the availability of many products in the market, dental caries and oral health problems still occur at high prevalence. Thus the introduction of natural remedies that can help promote oral health is of great importance. Eurycoma Longifolia Jack or Tongkat Ali roots have been known for a diverse spectrum of medicinal properties, one of which is antimicrobial activity. Thus our objectives were to test the activity of the root extract against important oral bacteria and fungi that are responsible for causing dental caries and other oral conditions. The roots of E. longifolia or Tongkat Ali have been used to in traditional medicine as well as supplements and food additives. Many chemical compounds have been detected in extracts of its roots which is believed to be responsible for its medicinal properties. In this study, our objectives were to study the effect of the E. Longifolia root extracts on the growth of Streptococcus Mutans, Lactobacillus and Candida Albicans isolated from saliva of adult patients with high caries risk. Materials and methods: The ethanolic extract of the root of this plant was tested against saliva isolated Streptococcus Mutans, Lactobacillus and Candida Albicans via disc diffusion assay at a concentration of 200mg/mL. The minimum inhibitory concentration was carried out by the standard broth microdilution method. Cell viability of test microorganisms against different concentration of the extract and inhibition zones were calculated. Results: Disk diffusion assay showed positive zones of inhibition for all test microorganisms with S. mutans, Lactobacillus and C. albicans exhibiting zones of inhibition of 8.3 ± 0.7mm, 12.4 ± 2.4mm and 21.4 ± 2.7mm respectively. For minimum inhibitory concentration, the test microorganisms were tested at concentration of 250mg/mL, 125mg/mL, 62.5mg/mL, 31.3mg/mL and 0mg/mL.The minimum inhibitory concentration showed that MIC of S. mutans was at 62.5mg/mL, Lactobacillus at 125mg/mL and C. albicans at 31.3mg/mL. Lastly, the cell viability results supports the MIC determined prior. Conclusion: Ethanol-based E. longifolia Jack root extract has an antimicrobial effect on the following microorganisms isolated from the saliva of high risk caries adult patients: S. mutans, Lactobacillus and C. albicans.