Myo-inositol ameliorative effects on cryo-survival of post-thawed abnormal human sperms quality towards infertility care
Background Sperm cryopreservation usually results in a significant decrease in sperm quality after thawing. The deleterious effects of freeze-thaw on abnormal sperm samples are considerably more severe. Various supplemental agents have been studied to ameliorate the negative effects due to cryopre...
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my.iium.irep.1065142023-09-11T09:46:32Z http://irep.iium.edu.my/106514/ Myo-inositol ameliorative effects on cryo-survival of post-thawed abnormal human sperms quality towards infertility care Abdul Wahab, Azantee Yazmie Ahmad Yusof, Zamri Abdul Rahman, Suzanah Md Isa, Muhammad Lokman Mazlan, Muhammad Alif Q Science (General) QM Human anatomy QP Physiology R Medicine (General) Background Sperm cryopreservation usually results in a significant decrease in sperm quality after thawing. The deleterious effects of freeze-thaw on abnormal sperm samples are considerably more severe. Various supplemental agents have been studied to ameliorate the negative effects due to cryopreservation. Myo-Inositol is likely to positively affect fertility. Therefore, this study aims to evaluate the beneficial effect of Myo-inositol (MI) supplementation in sperm freezing media for abnormal sperm. Methods Semen samples were collected from 23 male subjects that have abnormal sperm quality. Semen analysis has been conducted before freezing (pre-freeze). The samples were frozen with only a freezing medium (control) or MI-supplemented freezing medium. Semen analysis was re-evaluated after post-thaw for both groups. GoldCyto test kits were used in both post-thaw samples to evaluate the DNA fragmentation. Results The total motility, Progressive motility, morphology, and viability mean values of the sperms frozen with the MI-supplemented medium are greater compared to the control group (p<0.05). The post-thaw sample with MI supplementation (39.8%) has a higher cryo-survival rate (CSR) compared to the control group (23.4%). Conclusion MI supplementation in cryopreserved abnormal sperm samples significantly enhanced the post-thaw sperm motility, morphology, and vitality, and potentially increase the sperm survival rate. 2023 Article PeerReviewed application/pdf en http://irep.iium.edu.my/106514/7/106514_Myo-inositol%20ameliorative%20effects%20on%20cryo-survival%20of%20post-thawed%20abnormal%20human%20sperms%20quality%20towards%20infertility%20care.pdf Abdul Wahab, Azantee Yazmie and Ahmad Yusof, Zamri and Abdul Rahman, Suzanah and Md Isa, Muhammad Lokman and Mazlan, Muhammad Alif (2023) Myo-inositol ameliorative effects on cryo-survival of post-thawed abnormal human sperms quality towards infertility care. Journal of Reproductive Biotechnology and Fertility. ISSN 20589158 https://www.ivf-hub.net/journal-archive-new/2023-2/ |
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Q Science (General) QM Human anatomy QP Physiology R Medicine (General) Abdul Wahab, Azantee Yazmie Ahmad Yusof, Zamri Abdul Rahman, Suzanah Md Isa, Muhammad Lokman Mazlan, Muhammad Alif Myo-inositol ameliorative effects on cryo-survival of post-thawed abnormal human sperms quality towards infertility care |
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Background
Sperm cryopreservation usually results in a significant decrease in sperm quality after thawing. The deleterious effects of freeze-thaw on abnormal sperm samples are considerably more severe. Various supplemental agents have been studied to ameliorate the negative effects due to cryopreservation. Myo-Inositol is likely to positively affect fertility. Therefore, this study aims to evaluate the beneficial effect of Myo-inositol (MI) supplementation in sperm freezing media for abnormal sperm.
Methods
Semen samples were collected from 23 male subjects that have abnormal sperm quality. Semen analysis has been conducted before freezing (pre-freeze). The samples were frozen with only a freezing medium (control) or MI-supplemented freezing medium. Semen analysis was re-evaluated after post-thaw for both groups. GoldCyto test kits were used in both post-thaw samples to evaluate the DNA fragmentation.
Results
The total motility, Progressive motility, morphology, and viability mean values of the sperms frozen with the MI-supplemented medium are greater compared to the control group (p<0.05). The post-thaw sample with MI supplementation (39.8%) has a higher cryo-survival rate (CSR) compared to the control group (23.4%).
Conclusion
MI supplementation in cryopreserved abnormal sperm samples significantly enhanced the post-thaw sperm motility, morphology, and vitality, and potentially increase the sperm survival rate. |
format |
Article |
author |
Abdul Wahab, Azantee Yazmie Ahmad Yusof, Zamri Abdul Rahman, Suzanah Md Isa, Muhammad Lokman Mazlan, Muhammad Alif |
author_facet |
Abdul Wahab, Azantee Yazmie Ahmad Yusof, Zamri Abdul Rahman, Suzanah Md Isa, Muhammad Lokman Mazlan, Muhammad Alif |
author_sort |
Abdul Wahab, Azantee Yazmie |
title |
Myo-inositol ameliorative effects on cryo-survival of post-thawed abnormal human sperms quality towards infertility care |
title_short |
Myo-inositol ameliorative effects on cryo-survival of post-thawed abnormal human sperms quality towards infertility care |
title_full |
Myo-inositol ameliorative effects on cryo-survival of post-thawed abnormal human sperms quality towards infertility care |
title_fullStr |
Myo-inositol ameliorative effects on cryo-survival of post-thawed abnormal human sperms quality towards infertility care |
title_full_unstemmed |
Myo-inositol ameliorative effects on cryo-survival of post-thawed abnormal human sperms quality towards infertility care |
title_sort |
myo-inositol ameliorative effects on cryo-survival of post-thawed abnormal human sperms quality towards infertility care |
publishDate |
2023 |
url |
http://irep.iium.edu.my/106514/7/106514_Myo-inositol%20ameliorative%20effects%20on%20cryo-survival%20of%20post-thawed%20abnormal%20human%20sperms%20quality%20towards%20infertility%20care.pdf http://irep.iium.edu.my/106514/ https://www.ivf-hub.net/journal-archive-new/2023-2/ |
_version_ |
1778160473267503104 |
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13.160551 |