In vitro callus induction of Plukenetia Volubilis (Sacha Inchi), a PUFA-rich plant
The young leaves and ovules of Plukenetia volubilis were cultured in MS medium under 5 different treatments of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP) incubated in different conditions, either 24 hours light or 24 hours dark condition to develop an in vitro micropropagat...
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| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2021
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/100541/1/In%20vitro%20callus_Abstract.pdf http://irep.iium.edu.my/100541/ |
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| Summary: | The young leaves and ovules of Plukenetia volubilis were cultured in MS medium under 5 different treatments of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP) incubated in different conditions, either 24 hours light or 24 hours dark condition to develop an in vitro micropropagation through callus induction. Despite a series of contamination after surface sterilization, the young leaves explants deemed promising in developing callus in media supplemented with both plant growth regulators of 0.1 mg L-1 2,4-D + 0.05 mg L-1 BAP and 1.0 mg L-1 2,4-D + 0.05 mg L-1 BAP. Interestingly, it is the similar concentration that yielded callus from ovule. All treatments developed callus from ovule, in which the best callus was induced from MS media supplemented with 0.1 mg L-1 2,4- D + 0.05 mg L-1 BAP in both light and dark conditions compared to the single use of 2,4-D. This is the first report attempted on using ovules of sacha inchi as explant. |
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