N-hexane fraction of Bandotan Herb (Ageratum conyzoides L.) increasing Doxorubicin cytotoxic effect in MCF-7 breast cancer cells line

• Main Authors: Febriansyah, Rifky, Lubis, Windi Andriati, Bakhtiar, M. Taher
• Format: Article
• Language: English
• Published: EM International 2022
• Subjects: RM Therapeutics. Pharmacology
• Online Access: http://irep.iium.edu.my/100450/7/100450_N-hexane%20fraction%20of%20Bandotan%20Herb.pdf; http://irep.iium.edu.my/100450/; http://www.envirobiotechjournals.com/article_abstract.php?aid=12340&iid=348&jid=3

Breast cancer is one of the most common cancers that causes the highest morbidity and mortality in humans. Some anticancer medicines are not efficient and also have many side effects. Based on the Previous researches showed That bandotan herb extract contained several compounds which potentially have anti-microbial, anti-inflammatory and anti-cancer effects. This study aimed to analyze the cytotoxic activity and co- chemotherapy effect of n-hexane fraction of Bandotan (NFB) combined with doxorubicin in MCF-7 breast cancer cells line by in vitro assay and study the molecular mechanism of the active component NFB by in silico assay, with HER-2 as protein target. Antioxidant activity test of NFB measured by DPPH method and cytotoxicity assay of NFB performed with MTT assay method was carried out by molecular docking method between ageratochromene dimer (compound marker of bandotan) on HER-2 protein. Based on the research showed that 1 kg of Ageratum conyzoides L. herb powder obtained 1.5g NFB concentrated exctract which would then be utilized in antioxidant and cytotoxic test. The docking molecular method results showed NFB as a potential cytotoxic agent mediated by the ability of ageratochromene dimer inhibited HER-2 protein targets with docking scoreof -6.2 kcal/mol and doxorubicin as comparison with docking score of - 6.9 kcal/mol. Antioxidant activity of NFB showed the IC50value was 493 μg/ml. The NFB had low potency as citotoxic activity with IC50 value was 306 μg/ml to inhibit the growth of MCF-7 cells line but showed synergistic effect in combination with doxorubicin with Combination Index (CI) value between 0.53-0.90. From the result obtained it could be concluded that the NFB has weak potential as antioxidant and single cytotoxic activity but hassynergistic effect as co-chemotherapy when combined with doxorubicin on MCF- 7 breast cancer cells line.