Production of Chitosan Oligosaccharides using β-glycosidic degrading enzyme: optimization using response surface methodology
Many researchers have focused chitosan as a source of potential bioactive material during the past few decades. However, chitosan has several drawbacks to be utilised in biological applications, including poor solubility under physiological conditions. Therefore, a new interest has recently emerge...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Sultan Zainal Abidin
2020
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| Subjects: | |
| Online Access: | http://eprints.unisza.edu.my/7272/1/FH02-FBIM-20-43240.pdf http://eprints.unisza.edu.my/7272/ |
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| Summary: | Many researchers have focused chitosan as a source of potential bioactive material during the past few
decades. However, chitosan has several drawbacks to be utilised in biological applications, including poor
solubility under physiological conditions. Therefore, a new interest has recently emerged on partially
hydrolysed chitosan, chitosan oligosaccharides (COS). In this study, degradation of chitosan was performed
by Cellulase from Trichoderma reesei® 1.5L and Response Surface Methodology (RSM) were employed to
optimize the hydrolysis temperature, pH, enzyme concentration and substrate concentration. Optimization
of cellulase T. reesei® using central composite design (CCD) was to obtain optimum parameters and all the
factors showed significant effects (p˂0.05). The maximum response, Celluclast® activity (1.268 U) was
obtained by assaying the process at 49.79oC, pH 4.5, 3% (v/w) of enzyme concentration and 25% (w/v)
concentration of chitosan for 24 hours. |
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