Anti-proliferative effect of duku (Lansium Domesticum Corr) extracts on human colorectal adenocarcinoma cell lines

• Main Authors: Mohd Adzim Khalili, Rohin, Norhayati, Abd. Hadi, Wan Rohani, Wan Taib
• Format: Article
• Language: English
• Published: Research Journal of Pharmaceutical, Biological and Chemical Sciences 2016
• Subjects: R Medicine (General)
• Online Access: http://eprints.unisza.edu.my/7225/1/FH02-FSK-16-05741.jpg; http://eprints.unisza.edu.my/7225/

In the treatment or prevention of cancer, fruits and plants have a high content of natural antioxidants and the potential to protect humans against cancer. The duku fruit or Lansium Domesticum Corr, is rich in bioactive compounds and nutrients which important to health benefits. The purpose of this study is to screen possible anticancer activity of various extracts and fractions of duku fruit extracts that inhibit the proliferation of human colorectal adenocarcinoma (HT29). The duku fruit extract process involved three different solvents (methanol, ethanol and ethyl acetate). The human colorectal adenocarcinoma cell line was treated with concentration methanol, ethanol and ethyl acetate extracts (0-60μg/ml) from the duku fruit. The anti-proliferative activities of human colon adenocarcinoma cell line treated with duku fruit extracts were determined using MTT Assays (570nm). This study found that only the methanol extract had inhibited the proliferation activity of cancer cell lines. The degree of inhibited proliferation is defined as a concentration that reduced the number of cells to 50% compared to the untreated sample (IC50). Methanol extract inhibited the proliferative HT-29 cell lines at concentration of 6.8 μg/ml. Methanol extract from the duku fruit was found to be more successful in inhibiting HT29 cell proliferation compared to the Ethanol and Ethyl Acetate extracts.