SOX6, SOX13 and SOX9 expression pattern in meningioma in East Coast Malaysia
SOX (Sry-related high-mobility-group-box) family plays significant functions in human development from embryonic development, organogenesis and the most recent findings, tissue regeneration. As cell key modulators, deregulation of these genes has been associated with several human diseases includi...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://eprints.unisza.edu.my/6732/1/FH02-FSK-20-42737.pdf http://eprints.unisza.edu.my/6732/ |
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| Summary: | SOX (Sry-related high-mobility-group-box) family plays significant functions in human development from
embryonic development, organogenesis and the most recent findings, tissue regeneration. As cell key
modulators, deregulation of these genes has been associated with several human diseases including cancer.
SoxD and SoxE, two of the nine SOX subgroups and their molecular mechanism in carcinogenesis are widely
studied. Eventhough there are a considerable number of studies done on SOX gene in glioma, such studies in
meningioma, which is the most frequent brain tumor type in East Coast Malaysia are still lacking. Thus, this
study aimed to determine the expression levels of SOX6 (SoxD), SOX13 (SoxD) and SOX9 (SoxE) in both
WHO grade I and WHO grade II meningioma in Malaysian population. An FFPE sample of WHO grade I,
WHO grade II meningioma and a normal brain FFPE tissue were sectioned by using microtome. RNA
extraction were completed and the best quality and concentration of RNA were proceeded for cDNA
conversion before underwent gene expression analysis, real-time PCR (qPCR). The results displayed that
SOX6, SOX13 and SOX9 gene were downregulated in all low-grade meningioma compared to normal tissue.
In addition, there were upregulations observed in both SOX6 and SOX9 expressions but downregulated in
high-grade meningioma for SOX13. SOX6, SOX13 and SOX9 expression levels in selected brain tumours in
Malaysian population provide new insights of SoxD and SoxE expression in this population. The well-known
versatility functions of SOX genes and the canonical interaction of SOX genes with their co-factors may
elucidate the inconsistency of SOX gene expression level across diseases and genetic backgrounds. Thus,
functional studies are recommended to be carried out to observe the selected genes’ functions and mechanisms
whether they should reflect their diverse roles in specific Malaysian population. |
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