Detection of somaclonal variation in aquatic plant pearl grass (Hemianthus micranthemoides)

Hemianthus micranthemoides is an ornamental plan used for foreground plant in aquarium decorations. In Malaysia, the plant available on market is regenerated through tissue culture techniques which expose the risk for somaclonal variation. The objectives of this study are to identify suitable Rand...

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Main Authors: Nor Hasima, Mahmod, Tajul Afif, Abdullah, Nur Izyan, Sukimin
Format: Article
Language:English
Published: 2019
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Online Access:http://eprints.unisza.edu.my/6550/1/FH02-FBIM-19-32021.pdf
http://eprints.unisza.edu.my/6550/
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spelling my-unisza-ir.65502022-05-22T01:44:32Z http://eprints.unisza.edu.my/6550/ Detection of somaclonal variation in aquatic plant pearl grass (Hemianthus micranthemoides) Nor Hasima, Mahmod Tajul Afif, Abdullah Nur Izyan, Sukimin QH301 Biology Hemianthus micranthemoides is an ornamental plan used for foreground plant in aquarium decorations. In Malaysia, the plant available on market is regenerated through tissue culture techniques which expose the risk for somaclonal variation. The objectives of this study are to identify suitable Random Amplified Polymorphic DNA (RAPD) markers for determining somaclonal variation rate in micropropagated plantlets of H. micranthemoides and to observe the effects of somaclonal variation on the plantlet growth (area of spreadability, shoot and root length). Out of 20 RAPD primers screened by polymerase chain reaction (PCR) using mother plant, one was selected as a suitable marker to detect somaclonal variation of H. micranthemoides which was P15. Somaclonal variation rate of the H. micranthemoides was determined by analyzing the RAPD-PCR bands. The results showed somaclonal variation was observed at the fifth subculture (S5) with the percentage of polymorphic bands of 50 % when compared to mother plant. Repeated subclutures has affected the growth of the regenerates negatively. It was shown that as the number of subculture increased, the growth of plantlet decreased in terms of decreasing shoot and root length as well as area of spreadability. This findings serves as a guide especially for tissue culturist to minimize subculture numbers when micropropagating H. micranthemoides. 2019-12 Article PeerReviewed text en http://eprints.unisza.edu.my/6550/1/FH02-FBIM-19-32021.pdf Nor Hasima, Mahmod and Tajul Afif, Abdullah and Nur Izyan, Sukimin (2019) Detection of somaclonal variation in aquatic plant pearl grass (Hemianthus micranthemoides). Journal Of Agrobiotechnology, 10 (2). pp. 64-72. ISSN e-ISSN:2180-1983
institution Universiti Sultan Zainal Abidin
building UNISZA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sultan Zainal Abidin
content_source UNISZA Institutional Repository
url_provider https://eprints.unisza.edu.my/
language English
topic QH301 Biology
spellingShingle QH301 Biology
Nor Hasima, Mahmod
Tajul Afif, Abdullah
Nur Izyan, Sukimin
Detection of somaclonal variation in aquatic plant pearl grass (Hemianthus micranthemoides)
description Hemianthus micranthemoides is an ornamental plan used for foreground plant in aquarium decorations. In Malaysia, the plant available on market is regenerated through tissue culture techniques which expose the risk for somaclonal variation. The objectives of this study are to identify suitable Random Amplified Polymorphic DNA (RAPD) markers for determining somaclonal variation rate in micropropagated plantlets of H. micranthemoides and to observe the effects of somaclonal variation on the plantlet growth (area of spreadability, shoot and root length). Out of 20 RAPD primers screened by polymerase chain reaction (PCR) using mother plant, one was selected as a suitable marker to detect somaclonal variation of H. micranthemoides which was P15. Somaclonal variation rate of the H. micranthemoides was determined by analyzing the RAPD-PCR bands. The results showed somaclonal variation was observed at the fifth subculture (S5) with the percentage of polymorphic bands of 50 % when compared to mother plant. Repeated subclutures has affected the growth of the regenerates negatively. It was shown that as the number of subculture increased, the growth of plantlet decreased in terms of decreasing shoot and root length as well as area of spreadability. This findings serves as a guide especially for tissue culturist to minimize subculture numbers when micropropagating H. micranthemoides.
format Article
author Nor Hasima, Mahmod
Tajul Afif, Abdullah
Nur Izyan, Sukimin
author_facet Nor Hasima, Mahmod
Tajul Afif, Abdullah
Nur Izyan, Sukimin
author_sort Nor Hasima, Mahmod
title Detection of somaclonal variation in aquatic plant pearl grass (Hemianthus micranthemoides)
title_short Detection of somaclonal variation in aquatic plant pearl grass (Hemianthus micranthemoides)
title_full Detection of somaclonal variation in aquatic plant pearl grass (Hemianthus micranthemoides)
title_fullStr Detection of somaclonal variation in aquatic plant pearl grass (Hemianthus micranthemoides)
title_full_unstemmed Detection of somaclonal variation in aquatic plant pearl grass (Hemianthus micranthemoides)
title_sort detection of somaclonal variation in aquatic plant pearl grass (hemianthus micranthemoides)
publishDate 2019
url http://eprints.unisza.edu.my/6550/1/FH02-FBIM-19-32021.pdf
http://eprints.unisza.edu.my/6550/
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score 13.211869