The prevalence of unusual intramolecular thioester cross-link on the surface proteins of Enterococci
The emergence of nosocomial infections caused by multi-drug resistant enterococci is becoming a cause for concern. According to the 2015 National Surveillance of Antibiotic Resistance (NSAR) report by the Ministry of Health Malaysia, Enterococcus faecium was found to be resistant to a variety of a...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
2017
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| Subjects: | |
| Online Access: | http://eprints.unisza.edu.my/5804/1/FH02-FSK-18-13124.pdf http://eprints.unisza.edu.my/5804/ |
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| Summary: | The emergence of nosocomial infections caused by multi-drug resistant enterococci is becoming a cause for
concern. According to the 2015 National Surveillance of Antibiotic Resistance (NSAR) report by the Ministry
of Health Malaysia, Enterococcus faecium was found to be resistant to a variety of antibiotics especially to
vancomycin which recorded an increased resistant rate. Recent studies showed that many clinically significant
Grampositive human pathogens possess a unique covalent intramolecular thioester bond on at least one of their
surface 24dhesion proteins which may serve as potential therapeutic targets, an alternative to antibiotics.
Sequence search based on the protein query of thioester-containing domain (TED) of Streptococcus pyogenes
were performed using the Basic Local Alignment Search Tool (BLAST) against non-redundant protein
sequences (nr) and UniProt Knowledgebase (UniProtKB) databases. The protein BLAST followed by multiple
sequence alignment revealed that a total of 54 strains of E. faecium and 75 strains of E. faecalis were predicted to contain a thioester bond on their surface protein, whereby the critical cysteine and glutamine residues were
found to be perfectly conserved. These predicted TEDs showed low sequence homology (about 35 % similarity)
to known streptococcal TED and many of which functions are unknown. However, the high prevalence of TEDs
across enterococcal strains is astonishing. Therefore, the biochemical function of thioester bond in these proteins
contributing to enterococcal pathogenicity needs to be further assessed experimentally, which may serve as a
new drug target or diagnostic tool for enterococcal infections. |
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