Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets
Objective: To investigate antibiotic resistance profile and characterize Campylobacter jejuni (C. jejuni) isolates using random amplified polymorphic DNA (RAPD) analysis. Methods: Ninety eight C. jejuni isolates from farms and retail outlets were screened against 10 antibiotics commonly used clin...
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Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
2015
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Subjects: | |
Online Access: | http://eprints.unisza.edu.my/5071/1/FH02-FBIM-16-05225.pdf http://eprints.unisza.edu.my/5071/ |
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Summary: | Objective: To investigate antibiotic resistance profile and characterize Campylobacter
jejuni (C. jejuni) isolates using random amplified polymorphic DNA (RAPD) analysis.
Methods: Ninety eight C. jejuni isolates from farms and retail outlets were screened
against 10 antibiotics commonly used clinically and agriculturally by using disk diffusion
method. RAPD analysis was done to characterize 98 C. jejuni isolates.
Results: Fifty-one percent of the isolates had multiple antibiotic resistance index 0.2 and
below. This indicated that the isolates in the vegetables were not from the high risk
environment or extensive farming practices. C. jejuni isolates found resistant towards
penicillin G (93%), vancomycin (86%), ampicillin (35%), erythromycin (28%), gentamycin (4%), amikacin (3%), enrofloxacin (1%), norfloxacin (1%) and no resistance towards ciprofloxacin. RAPD clustering analysis showed that the contamination of C. jejuni
in vegetables was likely due to cross contamination at retail markets.
Conclusions: C. jejuni contamination in vegetables at retail markets was due to cross
contamination. Current finding proved that C. jejuni in small scale vegetables production
was less expose towards antibiotic abuse. |
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