Antibacterial activity and antioxidant capacity of Malaysian Tualang honey
Honey is known to possess therapeutic potential including antimicrobial activity and antioxidant. The aim of this study was to evaluate the antibacterial activity and antioxidant capacity of Malaysian tualang honey against five clinically important Gram positive and Gram negative bacteria. Broth d...
Saved in:
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
2015
|
Subjects: | |
Online Access: | http://eprints.unisza.edu.my/4900/1/FH02-FP-15-03566.pdf http://eprints.unisza.edu.my/4900/ |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | Honey is known to possess therapeutic potential including antimicrobial activity and antioxidant. The aim of this study was
to evaluate the antibacterial activity and antioxidant capacity of Malaysian tualang honey against five clinically important Gram positive
and Gram negative bacteria. Broth dilution method was used to evaluate the antibacterial activity of Tualang honey. Different
concentrations of honey (1.56mg/ml – 100mg/ml) in two-fold dilutions were tested against each bacterium. Minimum Inhibitory
Concentration (MIC) was determined by visual inspection and spectrophotometric (MIC95) readings at 620nm. Minimum bactericidal
concentration (MBC) was also obtained by culturing the bacteria on nutrient agar plates. The antioxidant capacity of Tualang honey
was also determined by estimating the total phenolic content, flavonoid content and radical scavenging activity (DPPH). The MIC of
Tualang honey by visual inspection and spectrophotometric (MIC95) reading ranged from 3.125mg/ml – 12.5mg/ml and 6.25mg/ml –
25mg/ml respectively. The MBCs values against the five tested bacteria ranged from 12.5mg/ml to 50mg/ml. The lowest and highest
MBCs values of Tualang honey were against S. enterica Serovar Typhimurium (12.5mg/ml) and E. coli (50mg/ml). The total phenolic
and flavonoids content of Tualang honey were found to be 275.6±12.5mg gallic acid/kg honey and 71.8±11.3mg quercetin/kg honey
respectively. The radical scavenging activity (DPPH) had inhibition concentration (IC50) of 460±16.3µg/ml. Malaysian tualang honey
has both bacteriostatic and bactericidal activities against clinically important Gram positive and Gram negative bacteria. The presence
of phenolics and flavonoids in the Tualang honey are responsible for its antioxidant capacity. |
---|