Implication and Genetic Variations on Lipid Profile of the Fasting Respondent
PPARs function as regulators of lipid and lipoprotein metabolism. The aim of the study was to compare the lipid profile between two phases of fasting and to examine the frequency and relationship of peroxisome proliferator-activated receptor, PPARα gene polymorphisms to lipid profile in fasti...
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my-unisza-ir.45682022-09-13T05:07:50Z http://eprints.unisza.edu.my/4568/ Implication and Genetic Variations on Lipid Profile of the Fasting Respondent Rohayu Izanwati, Mohd Rawi Abbe Maleyki, Mhd. Jalil Mohd Khairi, Zahri @ Johari Q Science (General) PPARs function as regulators of lipid and lipoprotein metabolism. The aim of the study was to compare the lipid profile between two phases of fasting and to examine the frequency and relationship of peroxisome proliferator-activated receptor, PPARα gene polymorphisms to lipid profile in fasting respondents. We conducted a case-control study protocol, which included 21 healthy volunteers without gender discrimination at the age of 18 years old. 3 ml of blood sample was drawn before the fasting phase and during the fasting phase (in Ramadhan month). 1ml of serum for the lipid profile was analyzed by using the automated chemistry analyser (Olympus, AU 400) and the data were analysed using the Paired T�Test (SPSS ver.20). DNA was extracted and PCR was conducted utilising 6 sets of primer. Primers were designed within 6 exons of interest in PPARα gene. Genetic and metabolic characteristics of fasting respondents and controls were estimated and compared. Fasting respondents were significantly have lowered the LDL levels (p=0.03). There were no polymorphisms detected except in exon 1 with 5% of this population study respectively. The polymorphisms in exon 1 of the PPARα gene were found in low frequency. Regarding the 1375G/T and 1386G/T polymorphisms in the exon 1 of the PPARα gene, the T-allele in fasting phase had no association with the decreased LDL levels (Fisher Exact Test). However this association is more promising when the sample size is larger in order to elucidate the precise impact of the polymorphisms on lipid profile in the population. In conclusion, the PPARα gene polymorphisms do not appear to affect the LDL of fasting respondents. 2013 Article PeerReviewed text en http://eprints.unisza.edu.my/4568/1/FH02-FPSK-14-01323.pdf Rohayu Izanwati, Mohd Rawi and Abbe Maleyki, Mhd. Jalil and Mohd Khairi, Zahri @ Johari (2013) Implication and Genetic Variations on Lipid Profile of the Fasting Respondent. International Journal of Medical, Pharmaceutical Science and Engineering, 7 (6). pp. 67-69. |
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Q Science (General) Rohayu Izanwati, Mohd Rawi Abbe Maleyki, Mhd. Jalil Mohd Khairi, Zahri @ Johari Implication and Genetic Variations on Lipid Profile of the Fasting Respondent |
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PPARs function as regulators of lipid and lipoprotein
metabolism. The aim of the study was to compare the lipid profile
between two phases of fasting and to examine the frequency and
relationship of peroxisome proliferator-activated receptor, PPARα
gene polymorphisms to lipid profile in fasting respondents. We
conducted a case-control study protocol, which included 21 healthy
volunteers without gender discrimination at the age of 18 years old. 3
ml of blood sample was drawn before the fasting phase and during
the fasting phase (in Ramadhan month). 1ml of serum for the lipid
profile was analyzed by using the automated chemistry analyser
(Olympus, AU 400) and the data were analysed using the Paired T�Test (SPSS ver.20). DNA was extracted and PCR was conducted
utilising 6 sets of primer. Primers were designed within 6 exons of
interest in PPARα gene. Genetic and metabolic characteristics of
fasting respondents and controls were estimated and compared.
Fasting respondents were significantly have lowered the LDL levels
(p=0.03). There were no polymorphisms detected except in exon 1
with 5% of this population study respectively. The polymorphisms in
exon 1 of the PPARα gene were found in low frequency. Regarding
the 1375G/T and 1386G/T polymorphisms in the exon 1 of the
PPARα gene, the T-allele in fasting phase had no association with the
decreased LDL levels (Fisher Exact Test). However this association
is more promising when the sample size is larger in order to elucidate
the precise impact of the polymorphisms on lipid profile in the
population. In conclusion, the PPARα gene polymorphisms do not
appear to affect the LDL of fasting respondents. |
format |
Article |
author |
Rohayu Izanwati, Mohd Rawi Abbe Maleyki, Mhd. Jalil Mohd Khairi, Zahri @ Johari |
author_facet |
Rohayu Izanwati, Mohd Rawi Abbe Maleyki, Mhd. Jalil Mohd Khairi, Zahri @ Johari |
author_sort |
Rohayu Izanwati, Mohd Rawi |
title |
Implication and Genetic Variations on Lipid Profile of the Fasting Respondent |
title_short |
Implication and Genetic Variations on Lipid Profile of the Fasting Respondent |
title_full |
Implication and Genetic Variations on Lipid Profile of the Fasting Respondent |
title_fullStr |
Implication and Genetic Variations on Lipid Profile of the Fasting Respondent |
title_full_unstemmed |
Implication and Genetic Variations on Lipid Profile of the Fasting Respondent |
title_sort |
implication and genetic variations on lipid profile of the fasting respondent |
publishDate |
2013 |
url |
http://eprints.unisza.edu.my/4568/1/FH02-FPSK-14-01323.pdf http://eprints.unisza.edu.my/4568/ |
_version_ |
1744358512570400768 |
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13.18916 |