Volatile Compounds in Effective Bait: Microbiological and Chemometric Approaches
House flies are commonly found at homes and business premises in Malaysia. They are known as vectors of pathogen because they carry the pathogenic bacteria such as Escherichia coli and salmonella. In doing so, various kinds of baits are used to attract and trap these flies either by mechanical or...
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my-unisza-ir.35382022-01-09T07:24:06Z http://eprints.unisza.edu.my/3538/ Volatile Compounds in Effective Bait: Microbiological and Chemometric Approaches Mohamed, Prof. Ts. Dr. Saiful Bahri Ghazali, Prof. Madya Dr. Puspa Liza Baig, Prof. Madya Dr. Atif Amin QR Microbiology TP Chemical technology House flies are commonly found at homes and business premises in Malaysia. They are known as vectors of pathogen because they carry the pathogenic bacteria such as Escherichia coli and salmonella. In doing so, various kinds of baits are used to attract and trap these flies either by mechanical or chemical means. But all these baits are not capable of attracting flies equally and its hygienic status is very important to human and pets when contacting directly with them. The main goal of this study is to find out the best bait that can attract house fly effectively and safely in every place, and its hygienic condition including effective time duration of the best bait performance. Experiments were conducted to determine and justify the best bait among six different baits commonly used in Malaysia. Two baits made in abroad and four baits made in Malaysia that are commonly used were selected for conducting this study. A simple, low-cost bottle trap technique was chosen to carry out the experiments in food industry, fish market and poultry farm in the east coast of Malaysia. The experimental data were used to determine the best bait. The hygienic condition of the best bait was determined by using a spread plate method under selective media such MRS agar, Eosin Methylene Blue (EMB) agar, and Dichloran Rose Bengal Chlortetract Cycline (DRBC) agar. Furthermore, Chemometric analysis (cluster analysis CA & discriminant analysis DA) and spread plate bacteria analysis on Nutrient Agar (NA) were done in order to determine the effective time for the best performance of the best bait. The spread plate bacteria analysis on NA was done for day 2, 3 and 4 only. Each time effective analysis was done by using eight discriminant variables: 30 minutes, 2 hours, 4 hours, 6 hours, 8 hours, 12 hours, control CEFCT powder, and control molasses (p < 0.05). It was found that Chinese Electronic Fly Catcher Trap (CEFCT) bait is the most effective one which can trap more house flies compared to other baits. It was also found through hygienic study that no significant growth of E.coli, Lactobacilus yeasts and moulds were formed in four days incubation period at 37o C. Hence, the CEFCT bait can be used safely. It was also observed that the CEFCT bait can be enabled its time effectiveness through CA and DA into three groups of time effectiveness given by correct classification (100%, 75%, and 75%). Bioassays analysis shows that CEFCT bait were very active up to 30 minutes through CFU count (9.28, 9.34, and 9.35 lofcfu/ml). The CEFCT bait is very much useful in trapping house flies and not harmful to human and animal health. Further studies are necessary to find low-cost bait components with similar effect of the best bait (CEFCT) in local markets in order to develop a new low cost bait. CreateSpace Independent Publishing Platform 2017 Book NonPeerReviewed text en http://eprints.unisza.edu.my/3538/1/FH05-FRIT-17-09625.pdf text en http://eprints.unisza.edu.my/3538/2/FH05-FRIT-17-09652.pdf text en http://eprints.unisza.edu.my/3538/3/FH05-FRIT-17-09624.pdf Mohamed, Prof. Ts. Dr. Saiful Bahri and Ghazali, Prof. Madya Dr. Puspa Liza and Baig, Prof. Madya Dr. Atif Amin (2017) Volatile Compounds in Effective Bait: Microbiological and Chemometric Approaches. CreateSpace Independent Publishing Platform. ISBN 978-1548762247 |
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QR Microbiology TP Chemical technology Mohamed, Prof. Ts. Dr. Saiful Bahri Ghazali, Prof. Madya Dr. Puspa Liza Baig, Prof. Madya Dr. Atif Amin Volatile Compounds in Effective Bait: Microbiological and Chemometric Approaches |
description |
House flies are commonly found at homes and business premises in Malaysia. They are
known as vectors of pathogen because they carry the pathogenic bacteria such as
Escherichia coli and salmonella. In doing so, various kinds of baits are used to attract
and trap these flies either by mechanical or chemical means. But all these baits are not
capable of attracting flies equally and its hygienic status is very important to human and
pets when contacting directly with them. The main goal of this study is to find out the
best bait that can attract house fly effectively and safely in every place, and its hygienic
condition including effective time duration of the best bait performance. Experiments
were conducted to determine and justify the best bait among six different baits
commonly used in Malaysia. Two baits made in abroad and four baits made in Malaysia
that are commonly used were selected for conducting this study. A simple, low-cost
bottle trap technique was chosen to carry out the experiments in food industry, fish
market and poultry farm in the east coast of Malaysia. The experimental data were used
to determine the best bait. The hygienic condition of the best bait was determined by
using a spread plate method under selective media such MRS agar, Eosin Methylene
Blue (EMB) agar, and Dichloran Rose Bengal Chlortetract Cycline (DRBC) agar.
Furthermore, Chemometric analysis (cluster analysis CA & discriminant analysis DA)
and spread plate bacteria analysis on Nutrient Agar (NA) were done in order to
determine the effective time for the best performance of the best bait. The spread plate
bacteria analysis on NA was done for day 2, 3 and 4 only. Each time effective analysis
was done by using eight discriminant variables: 30 minutes, 2 hours, 4 hours, 6 hours, 8
hours, 12 hours, control CEFCT powder, and control molasses (p < 0.05). It was found
that Chinese Electronic Fly Catcher Trap (CEFCT) bait is the most effective one which
can trap more house flies compared to other baits. It was also found through hygienic
study that no significant growth of E.coli, Lactobacilus yeasts and moulds were formed
in four days incubation period at 37o
C. Hence, the CEFCT bait can be used safely. It
was also observed that the CEFCT bait can be enabled its time effectiveness through
CA and DA into three groups of time effectiveness given by correct classification
(100%, 75%, and 75%). Bioassays analysis shows that CEFCT bait were very active up
to 30 minutes through CFU count (9.28, 9.34, and 9.35 lofcfu/ml). The CEFCT bait is
very much useful in trapping house flies and not harmful to human and animal health.
Further studies are necessary to find low-cost bait components with similar effect of the
best bait (CEFCT) in local markets in order to develop a new low cost bait. |
format |
Book |
author |
Mohamed, Prof. Ts. Dr. Saiful Bahri Ghazali, Prof. Madya Dr. Puspa Liza Baig, Prof. Madya Dr. Atif Amin |
author_facet |
Mohamed, Prof. Ts. Dr. Saiful Bahri Ghazali, Prof. Madya Dr. Puspa Liza Baig, Prof. Madya Dr. Atif Amin |
author_sort |
Mohamed, Prof. Ts. Dr. Saiful Bahri |
title |
Volatile Compounds in Effective Bait: Microbiological and Chemometric Approaches |
title_short |
Volatile Compounds in Effective Bait: Microbiological and Chemometric Approaches |
title_full |
Volatile Compounds in Effective Bait: Microbiological and Chemometric Approaches |
title_fullStr |
Volatile Compounds in Effective Bait: Microbiological and Chemometric Approaches |
title_full_unstemmed |
Volatile Compounds in Effective Bait: Microbiological and Chemometric Approaches |
title_sort |
volatile compounds in effective bait: microbiological and chemometric approaches |
publisher |
CreateSpace Independent Publishing Platform |
publishDate |
2017 |
url |
http://eprints.unisza.edu.my/3538/1/FH05-FRIT-17-09625.pdf http://eprints.unisza.edu.my/3538/2/FH05-FRIT-17-09652.pdf http://eprints.unisza.edu.my/3538/3/FH05-FRIT-17-09624.pdf http://eprints.unisza.edu.my/3538/ |
_version_ |
1724079271646855168 |
score |
13.160551 |